Western Blot (WB)
(Anti-GAD65 antibody, MBS175471, Western blottingLane 1: Rat Brain Tissue LysateLane 2: Rat Brain Tissue Lysate )
Rabbit GAD65 Polyclonal Antibody | anti-GAD2 antibody
Anti-GAD65 antibody
Western Blot (WB)
(Anti-GAD65 antibody, MBS175471, Western blottingLane 1: Rat Brain Tissue LysateLane 2: Rat Brain Tissue Lysate )
Western Blot (WB)
(Anti-GAD65 antibody, MBS175471, Western blottingLane 1: Rat Brain Tissue LysateLane 2: Rat Brain Tissue Lysate )
Immunohistochemistry (IHC)
(Anti-GAD65 antibody, MBS175471, IHC(P)IHC(P): Rat Brain Tissue )
Immunohistochemistry (IHC)
(Anti-GAD65 antibody, MBS175471, IHC(P)IHC(P): Rat Brain Tissue )
Background: Glutamate decarboxylase or glutamic acid decarboxylase(GAD) is an enzyme that catalyzes the decarboxylation of glutamate to GABA and CO2. GAD65 is the isoform with molecular weight of 65 kDa. Karlsen et al.(1991) mapped the GAD2 gene to chromosome 10p13-p11.2 by in situ hybridization. Bu et al.(1992) isolated a cDNA corresponding to the GAD2 gene from a human brain cDNA library. The deduced 585-amino acid protein has a molecular mass of 65 kD. GAD65 is target of autoantibodies in people who later develop type 1 diabetes mellitus or latent autoimmune diabetes.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2008]
Uniprot Description
GAD2: Catalyzes the production of GABA. Homodimer. Belongs to the group II decarboxylase family.
Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; EC 4.1.1.15; Carbohydrate Metabolism - butanoate; Lyase; Other Amino Acids Metabolism - taurine and hypotaurine; Other Amino Acids Metabolism - beta-alanine
Chromosomal Location of Human Ortholog: 10p11.23
Cellular Component: presynaptic membrane; Golgi membrane; synaptic vesicle membrane; axon; perinuclear region of cytoplasm; plasma membrane; cell junction; cytosol
Molecular Function: protein binding; glutamate binding; glutamate decarboxylase activity; protein heterodimerization activity; pyridoxal phosphate binding
Biological Process: response to drug; synaptic transmission; glutamate decarboxylation to succinate; neurotransmitter secretion; neurotransmitter biosynthetic process
Research Articles on GAD2
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Product Notes
The GAD2 gad2 (Catalog #AAA175471) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-GAD65 antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GAD65 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. Researchers should empirically determine the suitability of the GAD2 gad2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GAD65, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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