Rabbit anti-Mouse Vinculin Polyclonal Antibody | anti-VCL antibody

Anti-Vinculin antibody

Cat. Num. AAA175711

• Gene Names: VCL; MV; MVCL; CMD1W; CMH15; HEL114
• Reactivity: Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen affinity purified.
• Synonyms: Vinculin; Polyclonal Antibody; Anti-Vinculin antibody; vinculin; CMD1W antibody; CMH15 antibody; Metavinculin antibody; MVCL antibody; OTTHUMP00000019861 antibody; OTTHUMP00000019862 antibody; VCL antibody; VINC antibody; VINC_HUMAN antibody; Vinculin antibody; anti-VCL antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 1134

• Applicable Applications for anti-VCL antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Vinculin (173-188aa KMIDERQQELTHQEHR), identical to the related rat and mouse sequences.
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti-Vinculin antibody, MBS175711, Western blottingLane 1: Rat Heart Tissue LysateLane 2: Rat Brain Tissue LysateLane 3: Rat Liver Tissue LysateLane 4: U87 Cell LysateLane 5: SMMC Cell LysateLane 6: HEPA Cell LysateLane 7: HELA Cell LysateLane 8: HT1080 Cell Lysate)

Immunohistochemistry (IHC)

(Anti-Vinculin antibody, MBS175711, IHC(P)IHC(P): Human Mammary Cancer Tissue )

Immunohistochemistry (IHC)

(Anti-Vinculin antibody, MBS175711, IHC(P)IHC(P): Rat Intestine Tissue )

Related Product Information for anti-VCL antibody

Description: Rabbit IgG polyclonal antibody for Vinculin(VCL) detection. Tested with WB, IHC-P in Human, Mouse, Rat.
Background: In mammalian cells, vinculin is a membrane-cytoskeletal protein in focal adhesion plaques that is involved in linkage of integrin adhesion molecules to the actin cytoskeleton. By linkage studies in a 3-generation family, Mulligan et al.(1992) mapped the VCL gene to chromosome 10q22.1-q23, distal to D10S22.Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is though to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Multiple alternatively-spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. Human vinculin protein exhibits a greater-than-95% sequence identity to the chicken vinculin protein.Vinculin is a 117-kDa cytoskeletal protein with 1066 amino acids. The protein contains an acidic N-terminal domain and a basic C-terminal domain separated by a proline-rich middle segment. Vinculin consists of a globular head domain that contains binding sites for talin and alpha-actinin as well as a tyrosine phosphorylation site, while the tail region contains binding sites for F-actin, paxillin, and lipids.

NCBI and Uniprot Product Information

• NCBI GI #: 21903479
• NCBI GeneID: 7414
• UniProt Accession #: P18206; Q16450; Q5SWX2; Q7Z3B8; Q8IXU7
• Molecular Weight: 24,904 Da
• NCBI Official Full Name: Vinculin
• NCBI Official Synonym Full Names: vinculin
• NCBI Official Symbol: VCL
• NCBI Official Synonym Symbols: MV; MVCL; CMD1W; CMH15; HEL114
• NCBI Protein Information: vinculin; metavinculin; epididymis luminal protein 114
• UniProt Protein Name: Vinculin
• Protein Family: Vinculin
• UniProt Gene Name: VCL
• UniProt Synonym Gene Names: MV
• UniProt Entry Name: VINC_HUMAN

NCBI Description

Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

Vinculin: Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell- surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion. Exhibits self-association properties. Interacts with NRAP and SORBS1. Interacts with TLN1. Interacts with SYNM. Interacts with CTNNB1 and this interaction is necessary for its localization to the cell-cell junctions and for its function in regulating cell surface expression of E-cadherin. Metavinculin is muscle-specific. Belongs to the vinculin/alpha-catenin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal; Cell adhesion

Chromosomal Location of Human Ortholog: 10q22.2

Cellular Component: focal adhesion; costamere; protein complex; extracellular region; intercellular junction; fascia adherens; cytosol; actin cytoskeleton; cell-matrix junction; cell-cell adherens junction; adherens junction; cytoskeleton; plasma membrane; vesicle

Molecular Function: protein binding; cadherin binding; beta-catenin binding; structural molecule activity; actin binding; alpha-catenin binding

Biological Process: lamellipodium biogenesis; platelet activation; platelet degranulation; apical junction assembly; muscle contraction; axon extension; cell-matrix adhesion; morphogenesis of an epithelium; cell adhesion; cell motility; blood coagulation; negative regulation of cell migration

Disease: Cardiomyopathy, Dilated, 1w