Rabbit anti-Mouse, Rat PSGL-1/Selplg Polyclonal Antibody | anti-Selplg antibody
Anti-PSGL-1/Selplg Antibody
Gene Names
Selplg; CD162; Psgl1; Selp1; Selpl; Psgl-1
Reactivity
Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Immunogen affinity purified.
Synonyms
PSGL-1/Selplg; Polyclonal Antibody; Anti-PSGL-1/Selplg Antibody; P-selectin glycoprotein ligand 1; PSGL-1; Selectin P ligand; CD162; Selplg; Selp1; Selpl; anti-Selplg antibody
Host
Rabbit
Reactivity
Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
No cross reactivity with other proteins.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
397
Applicable Applications for anti-Selplg antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Direct ELISA (EIA)
Application Notes
WB: 0.25-0.5ug/ml (Mouse, Rat)
IHC-P (Embedded Section): 0.5-1ug/ml (Mouse, Rat)
Direct ELISA: 0.1-0.5ug/ml (Mouse)
Tested Species: In-house tested species with positive results.
IHC-P (Embedded Section): 0.5-1ug/ml (Mouse, Rat)
Direct ELISA: 0.1-0.5ug/ml (Mouse)
Tested Species: In-house tested species with positive results.
Immunogen
E Coli-derived mouse PSGL-1/Selplg recombinant protein (Position: L18-P397).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen store at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Related Product Information for anti-Selplg antibody
Description: Rabbit IgG polyclonal antibody for PSGL-1/Selplg detection. Tested with WB, IHC-P, Direct ELISA in Mouse; Rat.
Background: Selectin P ligand, also known as SELPLG or CD162 (cluster of differentiation 162), is a human gene. It is mapped to 5 F; 5 55.59 cM. This gene encodes a glycoprotein that functions as a high affinity counter-receptor for the cell adhesion molecules P-, E- and L- selectin expressed on myeloid cells and stimulated T lymphocytes. As such, this protein plays a critical role in leukocyte trafficking during inflammation by tethering of leukocytes to activated platelets or endothelia expressing selectins. This protein requires two post-translational modifications, tyrosine sulfation and the addition of the sialyl Lewis x tetrasaccharide (sLex) to its O-linked glycans, for its high-affinity binding activity. Aberrant expression of this gene and polymorphisms in this gene are associated with defects in the innate and adaptive immune response. Alternate splicing results in multiple transcript variants.
Background: Selectin P ligand, also known as SELPLG or CD162 (cluster of differentiation 162), is a human gene. It is mapped to 5 F; 5 55.59 cM. This gene encodes a glycoprotein that functions as a high affinity counter-receptor for the cell adhesion molecules P-, E- and L- selectin expressed on myeloid cells and stimulated T lymphocytes. As such, this protein plays a critical role in leukocyte trafficking during inflammation by tethering of leukocytes to activated platelets or endothelia expressing selectins. This protein requires two post-translational modifications, tyrosine sulfation and the addition of the sialyl Lewis x tetrasaccharide (sLex) to its O-linked glycans, for its high-affinity binding activity. Aberrant expression of this gene and polymorphisms in this gene are associated with defects in the innate and adaptive immune response. Alternate splicing results in multiple transcript variants.
References
1. Fuhlbrigge, R. C., Kieffer, J. D., Armerding, D., Kupper, T. S. Cutaneous lymphocyte antigen is a specialized form of PSGL-1 expressed on skin-homing T cells. Nature 389: 978-981, 1997.
2. Herron, M. J., Nelson, C. M., Larson, J., Snapp, K. R., Kansas, G. S., Goodman, J. L. Intracellular parasitism by the human granulocytic ehrlichiosis bacterium through the P-selectin ligand, PSGL-1. Science 288: 1653-1656, 2000.
3. Hidalgo, A., Weiss, L. A., Frenette, P. S. Functional selectin ligands mediating human CD34+ cell interactions with bone marrow endothelium are enhanced postnatally. J. Clin. Invest. 110: 559-569, 2002.
2. Herron, M. J., Nelson, C. M., Larson, J., Snapp, K. R., Kansas, G. S., Goodman, J. L. Intracellular parasitism by the human granulocytic ehrlichiosis bacterium through the P-selectin ligand, PSGL-1. Science 288: 1653-1656, 2000.
3. Hidalgo, A., Weiss, L. A., Frenette, P. S. Functional selectin ligands mediating human CD34+ cell interactions with bone marrow endothelium are enhanced postnatally. J. Clin. Invest. 110: 559-569, 2002.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
P-selectin glycoprotein ligand 1
NCBI Official Synonym Full Names
selectin, platelet (p-selectin) ligand
NCBI Official Symbol
Selplg
NCBI Official Synonym Symbols
CD162; Psgl1; Selp1; Selpl; Psgl-1
NCBI Protein Information
P-selectin glycoprotein ligand 1
UniProt Protein Name
P-selectin glycoprotein ligand 1
Protein Family
UniProt Gene Name
Selplg
UniProt Synonym Gene Names
Selp1; Selpl; PSGL-1
Uniprot Description
A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E- and P-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. Critical for the initial leukocyte capture.
Research Articles on Selplg
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Product Notes
The Selplg selplg (Catalog #AAA1753088) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PSGL-1/Selplg Antibody reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PSGL-1/Selplg can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Direct ELISA (EIA). WB: 0.25-0.5ug/ml (Mouse, Rat) IHC-P (Embedded Section): 0.5-1ug/ml (Mouse, Rat) Direct ELISA: 0.1-0.5ug/ml (Mouse) Tested Species: In-house tested species with positive results. Researchers should empirically determine the suitability of the Selplg selplg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PSGL-1/Selplg, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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