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Western Blot (WB) (PEG10 polyclonal antibody. Western Blot analysis of PEG10 expression in human stomach.)

Mouse anti-Human PEG10 Polyclonal Antibody

PEG10 (EDR, KIAA1051, MAR2, MART2, MEF3L1, RGAG3, Retrotransposon-derived Protein PEG10, Embryonal Carcinoma Differentiation-regulated Protein, Mammalian Retrotransposon-derived Protein 2, Myelin Expression Factor 3-like Protein 1, Paternally Expressed Ge

Reactivity
Human
Applications
Western Blot, Immunofluorescence
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Synonyms
PEG10; Polyclonal Antibody; PEG10 (EDR; KIAA1051; MAR2; MART2; MEF3L1; RGAG3; Retrotransposon-derived Protein PEG10; Embryonal Carcinoma Differentiation-regulated Protein; Mammalian Retrotransposon-derived Protein 2; Myelin Expression Factor 3-like Protein 1; Paternally Expressed Ge; Anti -PEG10 (EDR; anti-PEG10 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human PEG10.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Sequence
MTERRRDELSEEINNLREKVMKQSEENNNLQSQVQKLTEENTTLREQVEPTPEDEDDDIELRGAAAAAAPPPPIEEECPEDLPEKFDGNPDMLAPFMAQCQIFMEKSTRDFSVDRVRVCFVTSMMTGRAARWASAKLERSHYLMHNYPAFMMEMKHVFEDPQRREVAKRKIRRLRQGMGSVIDYSNAFQMIAQDLDWNEPALIDQYHEGLSDHIQEELSHLEVAKSLSALIGQCIHIERRLARAAAARKPRSPPRALVLPHIASHHQVDPTEPVGGARMRLTQEEKERRRKLNLCLYCGTGGHYADNCPAKASKSSPAGNSPAPL
Applicable Applications for anti-PEG10 antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes
Suitable for use in Immunofluorescence and Western Blot.
Dilution: Immunofluorescence: 10ug/ml
Immunogen
Full length human PEG10, aa1-325 (NP_001035242.1).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PEG10 polyclonal antibody. Western Blot analysis of PEG10 expression in human stomach.)

Western Blot (WB) (PEG10 polyclonal antibody. Western Blot analysis of PEG10 expression in human stomach.)

Western Blot (WB)

(Western Blot analysis of PEG10 expression in transfected 293T cell line by PEG10 polyclonal antibody. Lane 1: PEG10 transfected lysate (35.75kD). Lane 2: Non-transfected lysate.)

Western Blot (WB) (Western Blot analysis of PEG10 expression in transfected 293T cell line by PEG10 polyclonal antibody. Lane 1: PEG10 transfected lysate (35.75kD). Lane 2: Non-transfected lysate.)

Immunofluorescence (IF)

(Immunofluorescence of purified antibody to PEG10 on HeLa cell. [antibody concentration 10ug/ml])

Immunofluorescence (IF) (Immunofluorescence of purified antibody to PEG10 on HeLa cell. [antibody concentration 10ug/ml])
Related Product Information for anti-PEG10 antibody
This is a paternally expressed imprinted gene that encodes transcripts containing two overlapping open reading frames (ORFs), RF1 and RF1/RF2, as well as retroviral-like slippage and pseudoknot elements, which can induce a -1 nucleotide frame-shift. ORF1 encodes a shorter isoform with a CCHC-type zinc finger motif containing a sequence characteristic of gag proteins of most retroviruses and some retrotransposons. The longer isoform is the result of -1 translational frame-shifting leading to translation of a gag/pol-like protein combining RF1 and RF2. It contains the active-site consensus sequence of the protease domain of pol proteins. Additional isoforms resulting from alternatively spliced transcript variants, as well as from use of upstream non-AUG (CUG) start codon, have been reported for this gene. Increased expression of this gene is associated with hepatocellular carcinomas.
Product Categories/Family for anti-PEG10 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI Official Full Name
peg10

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Product Notes

The PEG10 (Catalog #AAA649728) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PEG10 (EDR, KIAA1051, MAR2, MART2, MEF3L1, RGAG3, Retrotransposon-derived Protein PEG10, Embryonal Carcinoma Differentiation-regulated Protein, Mammalian Retrotransposon-derived Protein 2, Myelin Expression Factor 3-like Protein 1, Paternally Expressed Ge reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PEG10 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). Suitable for use in Immunofluorescence and Western Blot. Dilution: Immunofluorescence: 10ug/ml. Researchers should empirically determine the suitability of the PEG10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MTERRRDELS EEINNLREKV MKQSEENNNL QSQVQKLTEE NTTLREQVEP TPEDEDDDIE LRGAAAAAAP PPPIEEECPE DLPEKFDGNP DMLAPFMAQC QIFMEKSTRD FSVDRVRVCF VTSMMTGRAA RWASAKLERS HYLMHNYPAF MMEMKHVFED PQRREVAKRK IRRLRQGMGS VIDYSNAFQM IAQDLDWNEP ALIDQYHEGL SDHIQEELSH LEVAKSLSAL IGQCIHIERR LARAAAARKP RSPPRALVLP HIASHHQVDP TEPVGGARMR LTQEEKERRR KLNLCLYCGT GGHYADNCPA KASKSSPAGN SPAPL. It is sometimes possible for the material contained within the vial of "PEG10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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