Rabbit anti-Human, Mouse EED Polyclonal Antibody | anti-EED antibody

EED (Embryonic ectoderm development, hEED, HEED, Polycomb protein EED, WAIT1, WAIT-1, WD protein associating with integrin cytoplasmic tails 1) (HRP)

Cat. Num. AAA6473534

• Gene Names: EED; HEED; WAIT1
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: EED; Polyclonal Antibody; EED (Embryonic ectoderm development; hEED; HEED; Polycomb protein EED; WAIT1; WAIT-1; WD protein associating with integrin cytoplasmic tails 1) (HRP); anti-EED antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human EED. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-EED antibody: Western Blot (WB)
• Application Notes: WB: 1ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to aa429-441 of human EED, conjugated to KLH. Species sequence homology: rat, opossum, chicken, canine, bovine.
• Conjugate: HRP
• Positive Control: Jurkat cell lysate, 3T3/A31 cell lysate
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot Analysis: Jurkat cell lysate was resolved by electrophoresis, transferred to PVDF and probed with 0.05 ug/ml anti-EED (1:20,000 dilution). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRPand a chemiluminescence detection system. Arrow indicates EED (62-70 kD))

Product Categories/Family for anti-EED antibody

Antibodies; Proteins

References

1. The Protein Data Bank, www.pdb.org, DOI 10.2210/pdb2qxv/pdb 2. Kuzmichev., A., et al. (2004). Mol Cell. 14(2):183-93. 3. Montgomery., ND., et al. (2005). Curr Biol. 15(10):942-7. 4. van der Vlag J, Otte AP (1999). Nat. Genet. 23 (4): 474-8. 5. Shibata., S., et al. (2008). EMBO J. 27(13):1816-26.

NCBI and Uniprot Product Information

• NCBI GI #: 24041020
• NCBI GeneID: 8726
• NCBI Accession #: NP_003788
• NCBI GenBank Nucleotide #: NM_003797.3
• UniProt Accession #: O75530; O00149; Q6NTH2; Q7LDA5; Q7LDG8; Q86VV2; Q9UNY7; A8K7V5
• Molecular Weight: 49 kDa
• NCBI Official Full Name: polycomb protein EED isoform a
• NCBI Official Synonym Full Names: embryonic ectoderm development
• NCBI Official Symbol: EED
• NCBI Official Synonym Symbols: HEED; WAIT1
• NCBI Protein Information: polycomb protein EED; WD protein associating with integrin cytoplasmic tails 1
• UniProt Protein Name: Polycomb protein EED
• Protein Family: Polycomb protein
• UniProt Gene Name: EED
• UniProt Synonym Gene Names: hEED; WAIT-1
• UniProt Entry Name: EED_HUMAN

NCBI Description

This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

EED: a member of the superfamily of Polycomb group (PcG) methyltransferase proteins with WD-40 repeats. PcG complexes mediate chromatin modifications that contribute to transcriptional silencing during development and carcinogenesis. A part of the Polycomb-repressive complex 2 (PRC2) along with EZH2, SUZ12, RBBP4 and RBBP7 and possibly AEBP2. Interacts with HDAC, HDAC2, histone H1 and YY1. PRC2 trimethylates histone H3 on K9 and K27, transcriptionally repressing target gene including HOXC8, HOXA9, MYT1 and CDKN2A. Appears to be overexpressed in breast and colon cancers. Inhibitors of PRC2-mediated gene repression are being investigated as a cancer therapy. EED and EZH2 are also implicated in the silencing of the inactive X chromosome. Three human isoforms produced by alternative splicing or initiation have been described, and additional isoforms may be produced by alternative initiation.

Protein type: Methyltransferase

Chromosomal Location of Human Ortholog: 11q14.2-q22.3

Cellular Component: nucleoplasm; sex chromatin; pronucleus; ESC/E(Z) complex; cytoplasm; nucleolus; nucleus

Molecular Function: histone methyltransferase activity; identical protein binding; protein binding; chromatin binding

Biological Process: histone methylation; genetic imprinting; transcription, DNA-dependent; negative regulation of gene expression, epigenetic; gene expression; negative regulation of transcription, DNA-dependent; regulation of gene expression, epigenetic