Rabbit anti-Mouse, Rat Aryl hydrocarbon Receptor/Ahr Polyclonal Antibody | anti-Ahr antibody

Anti-Aryl hydrocarbon Receptor/Ahr Antibody

Cat. Num. AAA1752918

• Reactivity: Mouse, Rat
• Applications: Western Blot, Flow Cytometry, Functional Assay, ELISA
• Purity: Immunogen affinity purified.
• Synonyms: Aryl hydrocarbon Receptor/Ahr; Polyclonal Antibody; Anti-Aryl hydrocarbon Receptor/Ahr Antibody; Aryl hydrocarbon receptor; Ah receptor; AhR; Ahr; anti-Ahr antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: No cross reactivity with other proteins.
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized; Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 853

• Applicable Applications for anti-Ahr antibody: Western Blot (WB), Flow Cytometry (FC/FACS), Direct ELISA (EIA)
• Application Notes: WB: 0.25-0.5ug/ml (Mouse, Rat); FC/FACS: 1-3ug/1x106 cells (Rat); Direct ELISA: 0.1-0.5ug/ml (Rat); Tested Species: In-house tested species with positive results.
• Immunogen: E Coli-derived rat Aryl hydrocarbon Receptor/Ahr recombinant protein (Position: R15-Q196).
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen store at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Flow Cytometry (FC/FACS)

Western Blot (WB)

(Figure 1. Western blot analysis of Ahr using anti-Ahr antibody.)

Related Product Information for anti-Ahr antibody

Description: Rabbit IgG polyclonal antibody for Aryl hydrocarbon Receptor/Ahr detection. Tested with WB, FCM, Direct ELISA in Mouse; Rat.

Background: AHR (aryl hydrocarbon receptor), also called bHLHe76, is a member of the family of basic helix-loop-helix transcription factors. AhR is a cytosolic transcription factor that is normally inactive, bound to several co-chaperones. The AHR gene is mapped on 7p21.1. Estrogenic actions of AHR agonists were detected in wildtype ovariectomized mouse uteri, but were absent in Ahr -/- or Er-alpha -/- ovariectomized mice. Complex assembly and ubiquitin ligase activity of CUL4B(AHR) in vitro and in vivo are dependent on the AHR ligand. In the CUL4B(AHR) complex, ligand-activated AHR acts as a substrate-specific adaptor component that targets sex steroid receptors for degradation. Cd4-positive cells from mice lacking Ahr developed Th17 responses but failed to produce Il22 and did not show enhanced Th17 development. Activation of Ahr during induction of EAE accelerated disease onset and increased pathology in wildtype mice, but not in Ahr -/- mice. The TDO-AHR pathway is active in human brain tumors and is associated with malignant progression and poor survival. Ahr activity within ROR-gamma-t-positive ILC could be induced by dietary ligands such as those contained in vegetables of the family Brassicaceae. 

References

1. Matikainen, T., Perez, G. I., Jurisicova, A., Pru, J. K., Schlezinger, J. J., Ryu, H.-Y., Laine, J., Sakai, T., Korsmeyer, S. J., Casper, R. F., Sherr, D. H., Tilly, J. L. Aromatic hydrocarbon receptor-driven Bax gene expression is required for premature ovarian failure caused by biohazardous environmental chemicals. Nature Genet. 28: 355-360, 2001.
2. Micka, J., Milatovich, A., Menon, A., Grabowski, G. A., Puga, A., Nebert, D. W. Human Ah receptor (AHR) gene: localization to 7p15 and suggestive correlation of polymorphism with CYP1A1 inducibility. Pharmacogenetics 7: 95-101, 1997.
3. Moura-Alves, P., Fae, K., Houthuys, E., Dorhoi, A., Kreuchwig, A., Furkert, J., Barison, N., Diehl, A., Munder, A., Constant, P., Skrahina, T., Guhlich-Bornhof, U., and 14 others. AhR sensing of bacterial pigments regulates antibacterial defence. Nature 512: 387-392, 2014.

NCBI and Uniprot Product Information

• NCBI GI #: 29337195
• NCBI GeneID: 25690
• UniProt Accession #: P41738
• NCBI Official Full Name: Aryl hydrocarbon receptor
• NCBI Official Synonym Full Names: aryl hydrocarbon receptor
• NCBI Official Symbol: Ahr
• NCBI Protein Information: aryl hydrocarbon receptor
• UniProt Protein Name: Aryl hydrocarbon receptor
• Protein Family: Aldehyde reductase
• UniProt Gene Name: Ahr
• UniProt Synonym Gene Names: Ah receptor; AhR

NCBI Description

binds 3-methylcholanthrene and other aromatic hydrocarbons; may play a role in bone formation [RGD, Feb 2006]

Uniprot Description

Ligand-activated transcriptional activator. Binds to the XRE promoter region of genes it activates. Activates the expression of multiple phase I and II xenobiotic chemical metabolizing enzyme genes (such as the CYP1A1 gene). Mediates biochemical and toxic effects of halogenated aromatic hydrocarbons. Involved in cell-cycle regulation. Likely to play an important role in the development and maturation of many tissues. Regulates the circadian clock by inhibiting the basal and circadian expression of the core circadian component PER1. Inhibits PER1 by repressing the CLOCK-ARNTL/BMAL1 heterodimer mediated transcriptional activation of PER1.