Western Blot (WB)
Rabbit anti-Mouse, Rat NUP214 Polyclonal Antibody | anti-Nup214 antibody
Anti-NUP214 Antibody
Gene Names
Nup214; CAN; BC039282; D2H9S46E
Reactivity
Mouse, Rat
Applications
Western Blot, Flow Cytometry, Functional Assay, ELISA
Purity
Immunogen affinity purified.
Synonyms
NUP214; Polyclonal Antibody; Anti-NUP214 Antibody; Nuclear pore complex protein Nup214; 214 kDa nucleoporin; Nucleoporin Nup214; Nup214; Kiaa0023; anti-Nup214 antibody
Host
Rabbit
Reactivity
Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
No cross reactivity with other proteins.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
2085
Applicable Applications for anti-Nup214 antibody
Western Blot (WB), Flow Cytometry (FC/FACS), Direct ELISA (EIA)
Application Notes
WB: 0.25-0.5ug/ml (Mouse, Rat)
FC/FACS: 1-3ug/1x106 cells (Mouse)
Direct ELISA: 0.1-0.5ug/ml (Mouse)
Tested Species: In-house tested species with positive results.
FC/FACS: 1-3ug/1x106 cells (Mouse)
Direct ELISA: 0.1-0.5ug/ml (Mouse)
Tested Species: In-house tested species with positive results.
Immunogen
E Coli-derived mouse NUP214 recombinant protein (Position: E987-N1137).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen store at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Western Blot (WB)
Western Blot (WB)
Related Product Information for anti-Nup214 antibody
Description: Rabbit IgG polyclonal antibody for NUP214 detection. Tested with WB, FCM, Direct ELISA in Mouse; Rat.
Background: Nucleoporin 214 (Nup2014) is a protein that in humans is encoded by the NUP214 gene. The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. This gene is a member of the FG-repeat-containing nucleoporins. The protein encoded by this gene is localized to the cytoplasmic face of the nuclear pore complex where it is required for proper cell cycle progression and nucleocytoplasmic transport. The 3' portion of this gene forms a fusion gene with the DEK gene on chromosome 6 in a t(6,9) translocation associated with acute myeloid leukemia and myelodysplastic syndrome. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.
Background: Nucleoporin 214 (Nup2014) is a protein that in humans is encoded by the NUP214 gene. The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. This gene is a member of the FG-repeat-containing nucleoporins. The protein encoded by this gene is localized to the cytoplasmic face of the nuclear pore complex where it is required for proper cell cycle progression and nucleocytoplasmic transport. The 3' portion of this gene forms a fusion gene with the DEK gene on chromosome 6 in a t(6,9) translocation associated with acute myeloid leukemia and myelodysplastic syndrome. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.
References
1. Kraemer D, Wozniak RW, Blobel G, Radu A (Feb 1994). "The human CAN protein, a putative oncogene product associated with myeloid leukemogenesis, is a nuclear pore complex protein that faces the cytoplasm". Proceedings of the National Academy of Sciences of the United States of America. 91 (4): 1519-23.
2. von Lindern M, Poustka A, Lerach H, Grosveld G (Aug 1990). "The (6;9) chromosome translocation, associated with a specific subtype of acute nonlymphocytic leukemia, leads to aberrant transcription of a target gene on 9q34". Molecular and Cellular Biology. 10 (8): 4016-26.
3. Napetschnig J, Blobel G, Hoelz A (2007). "Crystal structure of the N-terminal domain of the human protooncogene Nup214/CAN". Proc. Natl. Acad. Sci. U.S.A. 104 (6): 1783-8.
2. von Lindern M, Poustka A, Lerach H, Grosveld G (Aug 1990). "The (6;9) chromosome translocation, associated with a specific subtype of acute nonlymphocytic leukemia, leads to aberrant transcription of a target gene on 9q34". Molecular and Cellular Biology. 10 (8): 4016-26.
3. Napetschnig J, Blobel G, Hoelz A (2007). "Crystal structure of the N-terminal domain of the human protooncogene Nup214/CAN". Proc. Natl. Acad. Sci. U.S.A. 104 (6): 1783-8.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
nuclear pore complex protein Nup214
NCBI Official Synonym Full Names
nucleoporin 214
NCBI Official Symbol
Nup214
NCBI Official Synonym Symbols
CAN; BC039282; D2H9S46E
NCBI Protein Information
nuclear pore complex protein Nup214
UniProt Protein Name
Nuclear pore complex protein Nup214
Protein Family
UniProt Gene Name
Nup214
UniProt Synonym Gene Names
Kiaa0023
UniProt Entry Name
NU214_MOUSE
Uniprot Description
NUP214: May serve as a docking site in the receptor-mediated import of substrates across the nuclear pore complex. A chromosomal aberration involving NUP214 is found in a subset of acute myeloid leukemia (AML); also known as acute non-lymphocytic leukemia. Translocation t(6;9)(p23;q34) with DEK. It results in the formation of a DEK-CAN fusion gene. A chromosomal aberration involving NUP214 is found in some cases of acute undifferentiated leukemia (AUL). Translocation t(6;9)(q21;q34.1) with SET. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Oncoprotein; Nuclear export; Nucleoporin
Cellular Component: focal adhesion; intracellular membrane-bound organelle; cell; nuclear pore; intracellular; nucleus
Molecular Function: nucleocytoplasmic transporter activity
Biological Process: protein transport; mRNA export from nucleus; mRNA transport; protein import into nucleus; regulation of cell cycle; transport; protein export from nucleus
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Product Notes
The Nup214 nup214 (Catalog #AAA1753038) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-NUP214 Antibody reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NUP214 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Flow Cytometry (FC/FACS), Direct ELISA (EIA). WB: 0.25-0.5ug/ml (Mouse, Rat) FC/FACS: 1-3ug/1x106 cells (Mouse) Direct ELISA: 0.1-0.5ug/ml (Mouse) Tested Species: In-house tested species with positive results. Researchers should empirically determine the suitability of the Nup214 nup214 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NUP214, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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