Rabbit anti-Human, Mouse SLBP Polyclonal Antibody | anti-SLBP antibody

Anti-SLBP Antibody

Cat. Num. AAA1753060

• Gene Names: SLBP; HBP
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, ELISA
• Purity: Immunogen affinity purified.
• Synonyms: SLBP; Polyclonal Antibody; Anti-SLBP Antibody; Histone RNA hairpin-binding protein; Histone stem-loop-binding protein; HBP; anti-SLBP antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: No cross reactivity with other proteins.
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized; Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 270

• Applicable Applications for anti-SLBP antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Direct ELISA (EIA)
• Application Notes: WB: 0.25-0.5ug/ml (Human, Mouse); IHC-P (Embedded Section): 0.5-1ug/ml (Human, Mouse); ICC: 2ug/ml (Human); IF: 2ug/ml (Human); FC/FACS: 1-3ug/1x106 cells (Human); Direct ELISA: 0.1-0.5ug/ml (Human); Tested Species: In-house tested species with positive results.
• Immunogen: E Coli-derived human SLBP recombinant protein (Position: K146-S270).
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen store at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Flow Cytometry (FC/FACS)

Immunofluorescence (IF)

Immunohistochemistry (IHC)

Western Blot (WB)

(Figure 1. Western blot analysis of SLBP using anti-SLBP antibody.)

Related Product Information for anti-SLBP antibody

Description: Rabbit IgG polyclonal antibody for SLBP detection. Tested with WB, IHC-P, ICC/IF, FCM, Direct ELISA in Human;Mouse.

Background: Histone RNA hairpin-binding protein is a protein that in human is encoded by the SLBP gene. This gene is mapped to 4p16.3. This gene encodes a protein that binds to the stem-loop structure in replication-dependent histone mRNAs. Histone mRNAs do not contain introns or polyadenylation signals, and are processed by endonucleolytic cleavage. The stem-loop structure is essential for efficient processing but this structure also controls the transport, translation and stability of histone mRNAs. Expression of the protein is regulated during the cell cycle, increasing more than 10-fold during the latter part of G1.

References

1. Martin, F., Schaller, A., Eglite, S., Schumperli, D., Muller, B. The gene for histone RNA hairpin binding protein is located on human chromosome 4 and encodes a novel type of RNA binding protein. EMBO J. 16: 769-778, 1997.
2. Tan, D., Marzluff, W. F., Dominski, Z., Tong, L. Structure of histone mRNA stem-loop, human stem-loop binding protein, and 3-prime-hExo ternary complex. Science 339: 318-321, 2013.
3. Wang, Z.-F., Whitfield, M. L., Ingledue, T. C., III, Dominski, Z., Marzluff, W. F. The protein that binds the 3-prime end of histone mRNA: a novel RNA-binding protein required for histone pre-mRNA processing. Genes Dev. 10: 3028-3040, 1996.

NCBI and Uniprot Product Information

• NCBI GI #: 9789785
• NCBI GeneID: 7884
• UniProt Accession #: Q14493
• NCBI Official Full Name: Histone RNA hairpin-binding protein
• NCBI Official Synonym Full Names: stem-loop binding protein
• NCBI Official Symbol: SLBP
• NCBI Official Synonym Symbols: HBP
• NCBI Protein Information: histone RNA hairpin-binding protein
• UniProt Protein Name: Histone RNA hairpin-binding protein
• UniProt Gene Name: SLBP
• UniProt Synonym Gene Names: HBP
• UniProt Entry Name: SLBP_HUMAN

NCBI Description

This gene encodes a protein that binds to the stem-loop structure in replication-dependent histone mRNAs. Histone mRNAs do not contain introns or polyadenylation signals, and are processed by endonucleolytic cleavage. The stem-loop structure is essential for efficient processing but this structure also controls the transport, translation and stability of histone mRNAs. Expression of the protein is regulated during the cell cycle, increasing more than 10-fold during the latter part of G1. [provided by RefSeq, Jul 2008]