Rabbit anti-Mouse, Rat Growth hormone receptor/GHR Polyclonal Antibody | anti-Ghr antibody

Anti-Growth hormone receptor/GHR Antibody

Cat. Num. AAA1752948

• Gene Names: Ghr; GHBP; GHR/BP
• Reactivity: Mouse, Rat
• Applications: Western Blot, Flow Cytometry, Functional Assay, ELISA
• Purity: Immunogen affinity purified.
• Synonyms: Growth hormone receptor/GHR; Polyclonal Antibody; Anti-Growth hormone receptor/GHR Antibody; Growth hormone receptor; GH receptor; Somatotropin receptor; Growth hormone-binding protein; GH-binding protein; GHBP; Serum-binding protein; Ghr; anti-Ghr antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: No cross reactivity with other proteins.
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized; Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 650

• Applicable Applications for anti-Ghr antibody: Western Blot (WB), Flow Cytometry (FC/FACS), Direct ELISA (EIA)
• Application Notes: WB: 0.25-0.5ug/ml (Mouse, Rat); FC/FACS: 1-3ug/1x106 cells (Mouse, Rat); Direct ELISA: 0.1-0.5ug/ml (Mouse); Tested Species: In-house tested species with positive results.
• Immunogen: E Coli-derived mouse Growth hormone receptor/GHR recombinant protein (Position: T25-Q650).
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen store at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Flow Cytometry (FC/FACS)

Flow Cytometry (FC/FACS)

Western Blot (WB)

(Figure 1. Western blot analysis of Ghr using anti-Ghr antibody.)

Related Product Information for anti-Ghr antibody

Description: Rabbit IgG polyclonal antibody for Growth hormone receptor/GHR detection. Tested with WB, FCM, Direct ELISA in Mouse; Rat.

Background: Growth hormone receptor is a protein that in humans is encoded by the GHR gene. It is mapped to 15 A1; 15 1.84 cM. This gene encodes a member of the type I cytokine receptor family, which is a transmembrane receptor for growth hormone. Binding of growth hormone to the receptor leads to receptor dimerization and the activation of an intra- and intercellular signal transduction pathway leading to growth. Mutations in this gene have been associated with Laron syndrome, also known as the growth hormone insensitivity syndrome (GHIS), a disorder characterized by short stature. In humans and rabbits, but not rodents, growth hormone binding protein (GHBP) is generated by proteolytic cleavage of the extracellular ligand-binding domain from the mature growth hormone receptor protein. Multiple alternatively spliced transcript variants have been found for this gene.

References

1. Horan, M., Newsway, V., Yasmin, (NI), Lewis, M. D., Easter, T. E., Rees, D. A., Mahto, A., Millar, D. S., Procter, A. M., Scanlon, M. F., Wilkinson, I. B., Hall, I. P., Wheatley, A., Blakey, J., Bath, P. M. W., Cockcroft, J. R., Krawczak, M., Cooper, D. N. Genetic variation at the growth hormone (GH1) and growth hormone receptor (GHR) loci as a risk factor for hypertension and stroke. Hum. Genet. 119: 527-540, 2006.
2. Pantel, J., Grulich-Henn, J., Bettendorf, M., Strasburger, C. J., Heinrich, U., Amselem, S. Heterozygous nonsense mutation in exon 3 of the growth hormone receptor (GHR) in severe GH insensitivity (Laron syndrome) and the issue of the origin and function of the GHRd3 isoform. J. Clin. Endocr. Metab. 88: 1705-1710, 2003.
3. Tiulpakov, A., Rubstov, P., Dedov, I., Peterkova, V., Bezlepkina, O., Chrousos, G. P., Hochberg, Z. A novel C-terminal growth hormone receptor (GHR) mutation results in impaired GHR-STAT5 but normal STAT-3 signaling. J. Clin. Endocr. Metab. 90: 542-547, 2005.

NCBI and Uniprot Product Information

• NCBI GI #: 121178
• NCBI GeneID: 14600
• UniProt Accession #: P16882
• NCBI Official Full Name: Growth hormone receptor
• NCBI Official Synonym Full Names: growth hormone receptor
• NCBI Official Symbol: Ghr
• NCBI Official Synonym Symbols: GHBP; GHR/BP
• NCBI Protein Information: growth hormone receptor
• UniProt Protein Name: Growth hormone receptor
• Protein Family: Growth hormone receptor
• UniProt Gene Name: Ghr
• UniProt Synonym Gene Names: GH receptor; GH-binding protein; GHBP
• UniProt Entry Name: GHR_MOUSE

Uniprot Description

GH receptor: Receptor for pituitary gland growth hormone involved in regulating postnatal body growth. On ligand binding, couples to the JAK2/STAT5 pathway. Defects in GHR are a cause of Laron syndrome (LARS). A severe form of growth hormone insensitivity characterized by growth impairment, short stature, dysfunctional growth hormone receptor, and failure to generate insulin-like growth factor I in response to growth hormone. Defects in GHR may be a cause of idiopathic short stature autosomal (ISSA). Short stature is defined by a subnormal rate of growth. Belongs to the type I cytokine receptor family. Type 1 subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, cytokine; Membrane protein, integral

Cellular Component: extracellular space; extrinsic to membrane; cell surface; mitochondrion; cell soma; membrane; integral to plasma membrane; cytoplasm; integral to membrane; plasma membrane; extracellular region; nucleus; receptor complex

Molecular Function: growth hormone receptor activity; hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein binding; protein homodimerization activity; peptide hormone binding; growth factor binding; SH2 domain binding; protein phosphatase binding; protein kinase binding

Biological Process: activation of JAK protein; succinate metabolic process; oxaloacetate metabolic process; hormone-mediated signaling; activation of MAPK activity; positive regulation of JAK-STAT cascade; positive regulation of multicellular organism growth; tyrosine phosphorylation of JAK2 protein; fatty acid metabolic process; response to estradiol stimulus; 2-oxoglutarate metabolic process; allantoin metabolic process; creatinine metabolic process; isoleucine metabolic process; cytokine and chemokine mediated signaling pathway; valine metabolic process; citrate metabolic process; regulation of multicellular organism growth; endocytosis; JAK-STAT cascade; creatine metabolic process; multicellular organismal metabolic process; cellular response to hormone stimulus; positive regulation of peptidyl-tyrosine phosphorylation; insulin-like growth factor receptor signaling pathway; taurine metabolic process