Mouse Rab5/RAB5A Monoclonal Antibody | anti-RAB5A antibody
Anti-Rab5/RAB5A Antibody (monoclonal, 3E9)
Gene Names
RAB5A; RAB5
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Flow Cytometry, Functional Assay
Purity
Immunogen Affinity Purified
Synonyms
Rab5/RAB5A; Monoclonal Antibody; Anti-Rab5/RAB5A Antibody (monoclonal; 3E9); Ras-related protein Rab-5A; RAB5A; RAB5; member RAS oncogene family; anti-RAB5A antibody
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
3E9
Specificity
No cross reactivity with other proteins.
Purity/Purification
Immunogen Affinity Purified
Form/Format
Lyophilized
Sequence Length
201
Applicable Applications for anti-RAB5A antibody
Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes
WB: 0.1-0.5 mug/ml
FC/FACS: 1-3 mug/1x106 cells
Tested Species: In-house tested species with positive results.
FC/FACS: 1-3 mug/1x106 cells
Tested Species: In-house tested species with positive results.
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Rab5 (179-215aa KKLPKNEPQNPGANSARGRGVDLTEPTQPTRNQCCSN), different from the related mouse and rat sequences by three amino acids.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Relevant Detection Systems
It is recommended to use an Enhanced Chemiluminescent Kit with anti-Rabbit IgG (MBS176460) for Western blot.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -21 degree C for a longer time.
Avoid repeated freezing and thawing.
Avoid repeated freezing and thawing.
Related Product Information for anti-RAB5A antibody
Description: Mouse IgG monoclonal antibody for Rab5/RAB5A detection. Tested with Tested with WB, FCM in Human; Mouse; Rat.
Background: RAB5A (Ras-associated protein RAB5A), also called RAB5, is a protein that in humans is encoded by the RAB5A gene. RAB5 is a rate-limiting component of the machinery regulating the kinetics of membrane traffic in the early endocytic pathway. The RAB5A gene is mapped on 3p24.3. RAB5 is indispensable for a form of receptor tyrosine kinase-induced actin remodeling called circular ruffling. It signals to the actin cytoskeleton through RNTRE, a RAB5-specific GTPase-activating protein (GAP). RAB5 activity on phagosome membranes began to increase on disassembly of the actin coat encapsulating phagosomes. In addition, RAB5 activation is either continuous or repetitive for up to 10 minutes, but it ends before the collapse of engulfed apoptotic cells. Expression of a dominant-negative mutant of RAB5 delayed this collapse of apoptotic thymocytes, showing a role for RAB5 in phagosome maturation. RAB5A (Ras-associated protein RAB5A), also called RAB5, is a protein that in humans is encoded by the RAB5A gene. RAB5 is a rate-limiting component of the machinery regulating the kinetics of membrane traffic in the early endocytic pathway. The RAB5A gene is mapped on 3p24.3. RAB5 is indispensable for a form of receptor tyrosine kinase-induced actin remodeling called circular ruffling.?It signals to the actin cytoskeleton through RNTRE, a RAB5-specific GTPase-activating protein (GAP).?RAB5 activity on phagosome membranes began to increase on disassembly of the actin coat encapsulating phagosomes. In addition, RAB5 activation is either continuous or repetitive for up to 10 minutes, but it ends before the collapse of engulfed apoptotic cells. Expression of a dominant-negative mutant of RAB5 delayed this collapse of apoptotic thymocytes, showing a role for RAB5 in phagosome maturation.
Background: RAB5A (Ras-associated protein RAB5A), also called RAB5, is a protein that in humans is encoded by the RAB5A gene. RAB5 is a rate-limiting component of the machinery regulating the kinetics of membrane traffic in the early endocytic pathway. The RAB5A gene is mapped on 3p24.3. RAB5 is indispensable for a form of receptor tyrosine kinase-induced actin remodeling called circular ruffling. It signals to the actin cytoskeleton through RNTRE, a RAB5-specific GTPase-activating protein (GAP). RAB5 activity on phagosome membranes began to increase on disassembly of the actin coat encapsulating phagosomes. In addition, RAB5 activation is either continuous or repetitive for up to 10 minutes, but it ends before the collapse of engulfed apoptotic cells. Expression of a dominant-negative mutant of RAB5 delayed this collapse of apoptotic thymocytes, showing a role for RAB5 in phagosome maturation. RAB5A (Ras-associated protein RAB5A), also called RAB5, is a protein that in humans is encoded by the RAB5A gene. RAB5 is a rate-limiting component of the machinery regulating the kinetics of membrane traffic in the early endocytic pathway. The RAB5A gene is mapped on 3p24.3. RAB5 is indispensable for a form of receptor tyrosine kinase-induced actin remodeling called circular ruffling.?It signals to the actin cytoskeleton through RNTRE, a RAB5-specific GTPase-activating protein (GAP).?RAB5 activity on phagosome membranes began to increase on disassembly of the actin coat encapsulating phagosomes. In addition, RAB5 activation is either continuous or repetitive for up to 10 minutes, but it ends before the collapse of engulfed apoptotic cells. Expression of a dominant-negative mutant of RAB5 delayed this collapse of apoptotic thymocytes, showing a role for RAB5 in phagosome maturation.
References
1. Bucci, C., Parton, R. G., Mather, I. H., Stunnenberg, H., Simons, K., Hoflack, B., Zerial, M. The small GTPase rab5 functions as a regulatory factor in the early endocytic pathway. Cell 70: 715-728, 1992. 2.Kitano, M., Nakaya, M., Nakamura, T., Nagata, S., Matsuda, M. Imaging of Rab5 activity identifies essential regulators for phagosome maturation. Nature 453: 241-245, 2008. 3.Lanzetti, L., Rybin, V., Malabarba, M. G., Christoforidis, S., Scita, G., Zerial, M., Di Fiore, P. P. The Eps8 protein coordinates EGF receptor signalling through Rac and trafficking through Rab5. Nature 408: 374-377, 2000.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,178 Da
NCBI Official Full Name
ras-related protein Rab-5A isoform 2
NCBI Official Synonym Full Names
RAB5A, member RAS oncogene family
NCBI Official Symbol
RAB5A
NCBI Official Synonym Symbols
RAB5
NCBI Protein Information
ras-related protein Rab-5A
UniProt Protein Name
Ras-related protein Rab-5A
Protein Family
UniProt Gene Name
RAB5A
UniProt Synonym Gene Names
RAB5
Uniprot Description
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes (PubMed:10818110, PubMed:14617813, PubMed:16410077, PubMed:15378032). Contributes to the regulation of filopodia extension (PubMed:14978216). Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan (PubMed:22660413). Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3 ().
Research Articles on RAB5A
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Product Notes
The RAB5A rab5a (Catalog #AAA1752145) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Rab5/RAB5A Antibody (monoclonal, 3E9) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Rab5/RAB5A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Flow Cytometry (FC/FACS). WB: 0.1-0.5 mug/ml FC/FACS: 1-3 mug/1x106 cells Tested Species: In-house tested species with positive results. Researchers should empirically determine the suitability of the RAB5A rab5a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rab5/RAB5A, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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