Rabbit Rab5 Polyclonal Antibody | anti-Rab5 antibody

Rab5 Antibody

Cat. Num. AAA840388

• Gene Names: RAB5A; RAB5
• Reactivity: Human, Mouse, Rat, Monkey, Bovine
• Applications: Western Blot
• Purity: Affinity purified
• Synonyms: Rab5; Polyclonal Antibody; Rab5 Antibody; RAB5; Rab5a; RAS; RAS associated protein; RAB5A; RAB 5A; RAB 5; anti-Rab5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Monkey, Bovine
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: 100 ug (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.; Appearance: Colorless liquid
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 201

• Applicable Applications for anti-Rab5 antibody: Western Blot (WB)
• Application Notes: Western blotting (0.5-4 ug/ml), immunoprecipitation and immunhistochemistry. However, the optimal concentrations should be determined individually. The antibody recognizes 26 kDa Rab5 of human, mouse, monkey, bovine and rat origins. Reactivity to other species has not been tested.
• Immunogen: Synthetic peptide surrounding amino acid 185 of human Rab5
• Handling: The antibody solution should be gently mixed before use.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for anti-Rab5 antibody

Background: Rab5 is a 24 kDa GTP-binding protein that is localized to the cytoplasmic side of the plasma membrane, clathrin-coated vesicles, and early endosomes. Rab5 is believed to regulate vesicle fusion thromugh a cycle of GDP/GTP exchange and GTP hydrolysis. The different guanine nucleotide binding states of rab5 is postulated to affect its ability to associate or dissociate with membranes during endocytotic membrane traffic. Its GTP-bound form, which represents the active form of Rab5, associates with membrane and regulates vesicle docking and fusion.

NCBI and Uniprot Product Information

• NCBI GI #: 635574573
• NCBI GeneID: 5868
• NCBI Accession #: NP_001278977.1
• NCBI GenBank Nucleotide #: NM_001292048.1
• UniProt Accession #: P20339; Q6FI44; B4DJA5
• Molecular Weight: 22,178 Da
• NCBI Official Full Name: ras-related protein Rab-5A isoform 2
• NCBI Official Synonym Full Names: RAB5A, member RAS oncogene family
• NCBI Official Symbol: RAB5A
• NCBI Official Synonym Symbols: RAB5
• NCBI Protein Information: ras-related protein Rab-5A
• UniProt Protein Name: Ras-related protein Rab-5A
• Protein Family: Ras-related protein
• UniProt Gene Name: RAB5A
• UniProt Synonym Gene Names: RAB5
• UniProt Entry Name: RAB5A_HUMAN

Uniprot Description

RAB5A: Required for the fusion of plasma membranes and early endosomes. Contributes to the regulation of filopodia extension. Binds EEA1. Interacts with RIN1 and GAPVD1, which regulate its pathway, probably by acting as a GEF. Interacts with ALS2CL, RABEP1, SUN2, ZFYVE20 and RUFY1. Interacts with SGSM1 and SGSM3. Interacts with PIK3CB. Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP. Belongs to the small GTPase superfamily. Rab family.

Protein type: Motility/polarity/chemotaxis; G protein, monomeric, Rab; G protein; G protein, monomeric

Chromosomal Location of Human Ortholog: 3p24-p22

Cellular Component: zymogen granule membrane; protein complex; dendrite; early endosome; terminal button; phagocytic vesicle; cytosol; lipid raft; actin cytoskeleton; ruffle; synaptic vesicle; recycling endosome; cell soma; axon; perinuclear region of cytoplasm; endocytic vesicle; cytoplasm; plasma membrane; melanosome; endosome membrane; nerve terminal; endosome

Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding; GDP-dissociation inhibitor binding

Biological Process: regulation of long-term neuronal synaptic plasticity; intracellular protein transport; regulation of endosome size; early endosome to late endosome transport; positive regulation of smooth muscle cell proliferation; regulation of endocytosis; regulation of filopodium formation; endocytosis; blood coagulation; Rab protein signal transduction; positive regulation of smooth muscle cell migration; positive regulation of exocytosis