Human RAB5A, Member RAS Oncogene Family (RAB5A) RTU ELISA Kit | RAB5A rtu elisa kit

Human RAB5A, Member RAS Oncogene Family (RAB5A) ELISA Kit

Cat. Num. AAA4502154

• Gene Names: RAB5A; RAB5
• Reactivity: Human
• Synonyms: RAB5A; Member RAS Oncogene Family (RAB5A); Human RAB5A; Member RAS Oncogene Family (RAB5A) ELISA Kit; RAB5; RAB5A rtu elisa kit

• Reactivity: Human
• Sequence Length: 201

• Assay Type: Sandwich
• Samples: Tissue Homogenates or Other Biological Fluids.
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.122ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RAB5A rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to RAB5A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to RAB5A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RAB5A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of RAB5A in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 635574573
• NCBI GeneID: 5868
• NCBI Accession #: NP_001278977.1
• NCBI GenBank Nucleotide #: NM_001292048.1
• UniProt Accession #: P20339; Q6FI44; B4DJA5
• Molecular Weight: 22,178 Da
• NCBI Official Full Name: ras-related protein Rab-5A isoform 2
• NCBI Official Synonym Full Names: RAB5A, member RAS oncogene family
• NCBI Official Symbol: RAB5A
• NCBI Official Synonym Symbols: RAB5
• NCBI Protein Information: ras-related protein Rab-5A
• UniProt Protein Name: Ras-related protein Rab-5A
• Protein Family: Ras-related protein
• UniProt Gene Name: RAB5A
• UniProt Synonym Gene Names: RAB5

Uniprot Description

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes (PubMed:10818110, PubMed:14617813, PubMed:16410077, PubMed:15378032). Contributes to the regulation of filopodia extension (PubMed:14978216). Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan (PubMed:22660413). Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3 ().