Immunohistochemistry (IHC)
(Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE))
Rabbit anti-Human RPS3/Ribosomal Protein S3 Polyclonal Antibody | anti-RPS3 antibody
Anti-RPS3/Ribosomal Protein S3 Antibody
IHC-P: 5 ug/ml
IP: 1:500-1:5000
WB: 1:500-1:5000
Long term: -20 degree C.
Avoid freeze-thaw cycles.
Immunohistochemistry (IHC)
(Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE))
Immunohistochemistry (IHC)
(Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE))
Western Blot (WB)
(Western blot analysis using anti-RPS3 (human), pAb at 1:1000 dilution. 1: Vector alone-transfected HEK 293 cell lysate (V). 2: RPS3 (DYKDDDDK-tagged)-transfected HEK 293 cell lysate (FS3).)
Western Blot (WB)
(Western blot analysis using anti-RPS3 (human), pAb at 1:1000 dilution. 1: Vector alone-transfected HEK 293 cell lysate (V). 2: RPS3 (DYKDDDDK-tagged)-transfected HEK 293 cell lysate (FS3).)
NCBI and Uniprot Product Information
NCBI Description
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit, where it forms part of the domain where translation is initiated. The protein belongs to the S3P family of ribosomal proteins. Studies of the mouse and rat proteins have demonstrated that the protein has an extraribosomal role as an endonuclease involved in the repair of UV-induced DNA damage. The protein appears to be located in both the cytoplasm and nucleus but not in the nucleolus. Higher levels of expression of this gene in colon adenocarcinomas and adenomatous polyps compared to adjacent normal colonic mucosa have been observed. This gene is co-transcribed with the small nucleolar RNA genes U15A and U15B, which are located in its first and fifth introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]
Uniprot Description
Involved in translation as a component of the 40S small ribosomal subunit (PubMed:8706699). Has endonuclease activity and plays a role in repair of damaged DNA (PubMed:7775413). Cleaves phosphodiester bonds of DNAs containing altered bases with broad specificity and cleaves supercoiled DNA more efficiently than relaxed DNA (PubMed:15707971). Displays high binding affinity for 7,8-dihydro-8-oxoguanine (8-oxoG), a common DNA lesion caused by reactive oxygen species (ROS) (PubMed:14706345). Has also been shown to bind with similar affinity to intact and damaged DNA (PubMed:18610840). Stimulates the N-glycosylase activity of the base excision protein OGG1 (PubMed:15518571). Enhances the uracil excision activity of UNG1 (PubMed:18973764). Also stimulates the cleavage of the phosphodiester backbone by APEX1 (PubMed:18973764). When located in the mitochondrion, reduces cellular ROS levels and mitochondrial DNA damage (PubMed:23911537). Has also been shown to negatively regulate DNA repair in cells exposed to hydrogen peroxide (PubMed:17049931). Plays a role in regulating transcription as part of the NF-kappa-B p65-p50 complex where it binds to the RELA/p65 subunit, enhances binding of the complex to DNA and promotes transcription of target genes (PubMed:18045535). Represses its own translation by binding to its cognate mRNA (PubMed:20217897). Binds to and protects TP53/p53 from MDM2-mediated ubiquitination (PubMed:19656744). Involved in spindle formation and chromosome movement during mitosis by regulating microtubule polymerization (PubMed:23131551). Involved in induction of apoptosis through its role in activation of CASP8 (PubMed:14988002). Induces neuronal apoptosis by interacting with the E2F1 transcription factor and acting synergistically with it to up-regulate pro-apoptotic proteins BCL2L11/BIM and HRK/Dp5 (PubMed:20605787). Interacts with TRADD following exposure to UV radiation and induces apoptosis by caspase-dependent JNK activation (PubMed:22510408).
Research Articles on RPS3
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Product Notes
The RPS3 rps3 (Catalog #AAA2401794) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-RPS3/Ribosomal Protein S3 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RPS3/Ribosomal Protein S3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Immunoprecipitation (IP), Western Blot (WB). ELISA: 1:2000-1:5000 IHC-P: 5 ug/ml IP: 1:500-1:5000 WB: 1:500-1:5000. Researchers should empirically determine the suitability of the RPS3 rps3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RPS3/Ribosomal Protein S3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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