Rabbit Rab5 Polyclonal Antibody | anti-RAB5A antibody

Anti-Rab5 antibody

Cat. Num. AAA175653

• Gene Names: RAB5A; RAB5
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen affinity purified.
• Synonyms: Rab5; Polyclonal Antibody; Anti-Rab5 antibody; Ras-related protein Rab-5A; RAB5A; member RAS oncogene family; RAB 5 antibody; RAB 5A antibody; RAB5A antibody; RAB5A member RAS oncogene family antibody; RAB5A_HUMAN antibody; RAS associated protein RAB5A antibody; Ras related protein Rab 5A antibody; Ras-related protein Rab-5A antibody; anti-RAB5A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 215

• Applicable Applications for anti-RAB5A antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Rab5 (138-155aa ANKRAVDFQEAQSYADDN), identical to the related rat and mouse sequences.
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti-Rab5 antibody, MBS175653, Western blottingLane 1: Rat Brain Tissue LysateLane 2: Rat Ovary Tissue LysateLane 3: Human Placenta Tissue LysateLane 4: HELA Cell LysateLane 5: 293T Cell LysateLane 6: A375 Cell LysateLane 7: COLO320 Cell LysateLane 8: MM453 Cell Lysate )

Immunohistochemistry (IHC)

(Anti-Rab5 antibody, MBS175653, IHC(P)IHC(P):Rat Brain Tissue )

Related Product Information for anti-RAB5A antibody

Description: Rabbit IgG polyclonal antibody for Ras-related protein Rab-5A(RAB5A) detection. Tested with WB, IHC-P in Human, Mouse, Rat.
Background: RAB5A(Ras-associated protein RAB5A), also called RAB5, is a protein that in humans is encoded by the RAB5A gene. RAB5 is a rate-limiting component of the machinery regulating the kinetics of membrane traffic in the early endocytic pathway. The RAB5A gene is mapped on 3p24.3. Tuberin functions as a RAB5GAP in vivo to negatively regulate RAB5-GTP activity in endocytosis. Ectopically expressed ALS2 localized with RAB5 and early endosome antigen-1 onto early endosomal compartments and stimulated the enlargement of endosomes in cultured cortical neurons. RAB5 is indispensable for a form of receptor tyrosine kinase-induced actin remodeling called circular ruffling. RAB5 signals to the actin cytoskeleton through RNTRE, a RAB5-specific GTPase-activating protein(GAP). RAB5 activity on phagosome membranes began to increase on disassembly of the actin coat encapsulating phagosomes. RAB5 activation was either continuous or repetitive for up to 10 minutes, but it ended before the collapse of engulfed apoptotic cells. Expression of a dominant-negative mutant of RAB5 delayed this collapse of apoptotic thymocytes, showing a role for RAB5 in phagosome maturation.

NCBI and Uniprot Product Information

• NCBI GI #: 1346958
• NCBI GeneID: 5868
• UniProt Accession #: P20339; Q6FI44; B4DJA5
• Molecular Weight: 22,178 Da
• NCBI Official Full Name: Ras-related protein Rab-5A
• NCBI Official Synonym Full Names: RAB5A, member RAS oncogene family
• NCBI Official Symbol: RAB5A
• NCBI Official Synonym Symbols: RAB5
• NCBI Protein Information: ras-related protein Rab-5A; RAS-associated protein RAB5A
• UniProt Protein Name: Ras-related protein Rab-5A
• Protein Family: Ras-related protein
• UniProt Gene Name: RAB5A
• UniProt Synonym Gene Names: RAB5
• UniProt Entry Name: RAB5A_HUMAN

Uniprot Description

RAB5A: Required for the fusion of plasma membranes and early endosomes. Contributes to the regulation of filopodia extension. Binds EEA1. Interacts with RIN1 and GAPVD1, which regulate its pathway, probably by acting as a GEF. Interacts with ALS2CL, RABEP1, SUN2, ZFYVE20 and RUFY1. Interacts with SGSM1 and SGSM3. Interacts with PIK3CB. Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP. Belongs to the small GTPase superfamily. Rab family.

Protein type: G protein, monomeric, Rab; Motility/polarity/chemotaxis; G protein, monomeric; G protein

Chromosomal Location of Human Ortholog: 3p24-p22

Cellular Component: zymogen granule membrane; protein complex; early endosome; dendrite; terminal button; phagocytic vesicle; cytosol; actin cytoskeleton; lipid raft; ruffle; synaptic vesicle; recycling endosome; cell soma; endocytic vesicle; perinuclear region of cytoplasm; axon; cytoplasm; melanosome; plasma membrane; endosome membrane; nerve terminal; endosome

Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding; GDP-dissociation inhibitor binding

Biological Process: regulation of long-term neuronal synaptic plasticity; intracellular protein transport; regulation of endosome size; early endosome to late endosome transport; positive regulation of smooth muscle cell proliferation; regulation of endocytosis; endocytosis; regulation of filopodium formation; blood coagulation; positive regulation of smooth muscle cell migration; Rab protein signal transduction; positive regulation of exocytosis