Rabbit anti-Human SCUBE1 Polyclonal Antibody | anti-SCUBE1 antibody

SCUBE1 Antibody

Cat. Num. AAA151427

• Reactivity: Human
• Applications: ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: SCUBE1 Antibody is affinity chromatography purified via peptide column.
• Synonyms: SCUBE1; Polyclonal Antibody; SCUBE1 Antibody; Signal peptide; CUB and EGF-like domain-containing protein 1; signal peptide; CUB domain; EGF-like 1; anti-SCUBE1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: SCUBE1 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 988

• Applicable Applications for anti-SCUBE1 antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: SCUBE1 antibody can be used for detection of SCUBE1 by Western blot at 1 mug/mL. Antibody can also be used for immunocytochemistry starting at 20 mug/mL. For immunofluorescence start at 20 ug/mL.; Optimal dilutions for each application to be determined by the researcher.
• Conjugate: Unconjugated
• Immunogen: SCUBE1 antibody was raised against a 14 amino acid synthetic peptide near the center of human SCUBE1.
• Buffer: SCUBE1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Homology: Predicted species reactivity based on immunogen sequence: Mouse (93%)
• Positive control: Daudi Cell Lysate (Catalog Number: MBS151632)
• Preparation and Storage: SCUBE1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of SCUBE1 in Daudi cell lysate with SCUBE1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.)

Immunocytochemistry (IHC)

(Immunocytochemistry of SCUBE1 in Daudi cells with SCUBE1 antibody at 20 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of SCUBE1 in Daudi cells with SCUBE1 antibody at 20 μg/mL.)

Related Product Information for anti-SCUBE1 antibody

SCUBE1 Antibody: SCUBE1 is an EGF-related protein with a CUB (C1s-like) domain that is expressed primarily in the developing gonad, nervous system, somites, surface ectoderm and limb buds. The N-terminal EGF-like repeats of SCUBE1 participate in the formation of adhesions in a Ca2+-dependent fashion. In contrast, the C-terminal cysteine-rich motifs and CUB domain can directly bind and antagonize bone morphogenic protein activity; genetic ablation of this region resulted in brain malformation in mice embryos, suggesting SCUBE1 plays a role in brain morphogenesis. SCUBE1 has also been implicated in early craniofacial development in mice.

NCBI and Uniprot Product Information

• NCBI GI #: 120587029
• NCBI GeneID: 80274
• NCBI Accession #: NP_766638
• NCBI GenBank Nucleotide #: NM_173050.3
• UniProt Accession #: Q8IWY4; Q5R336
• Molecular Weight: 107,910 Da
• NCBI Official Full Name: signal peptide, CUB and EGF-like domain-containing protein 1
• NCBI Official Synonym Full Names: signal peptide, CUB domain, EGF-like 1
• NCBI Official Symbol: SCUBE1
• NCBI Protein Information: signal peptide, CUB and EGF-like domain-containing protein 1; signal peptide-CUB domain-EGF-related 1
• UniProt Protein Name: Signal peptide, CUB and EGF-like domain-containing protein 1
• Protein Family: Signal peptide, CUB and EGF-like domain-containing protein
• UniProt Gene Name: SCUBE1
• UniProt Entry Name: SCUB1_HUMAN

NCBI Description

This gene encodes a cell surface glycoprotein that is a member of the SCUBE (signal peptide, CUB domain, EGF (epidermal growth factor)-like protein) family. Family members have an amino-terminal signal peptide, nine copies of EGF-like repeats and a CUB domain at the carboxyl terminus. This protein is expressed in platelets and endothelial cells and may play an important role in vascular biology. [provided by RefSeq, Oct 2011]

Uniprot Description

SCUBE1: Could function as an adhesive molecule and its matrix bound and soluble fragments may play a critical role in vascular biology.

Chromosomal Location of Human Ortholog: 22q13

Cellular Component: extrinsic to plasma membrane; extracellular space; cell surface; external side of plasma membrane

Molecular Function: identical protein binding; protein heterodimerization activity; calcium ion binding

Biological Process: adult heart development; endothelial cell differentiation; blood coagulation; inflammatory response; protein homooligomerization; post-embryonic development