Rat Signal Peptide, CUB Domain, EGF Like 1 (SCUBE1) ELISA Kit | SCUBE1 elisa kit

Rat Signal Peptide, CUB Domain, EGF Like 1 (SCUBE1) ELISA Kit

Cat. Num. AAA2707804

• Reactivity: Rat
• Synonyms: Signal Peptide; CUB Domain; EGF Like 1 (SCUBE1); Rat Signal Peptide; EGF Like 1 (SCUBE1) ELISA Kit; SCUBE1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Signal Peptide, CUB Domain, EGF Like 1 (SCUBE1).; No significant cross-reactivity or interference between Signal Peptide, CUB Domain, EGF Like 1 (SCUBE1) and analogues was observed.
• Sequence Length: 988

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 78-5,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 32pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Signal Peptide, CUB Domain, EGF Like 1 (SCUBE1) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Signal Peptide, CUB Domain, EGF Like 1 (SCUBE1) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SCUBE1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Signal Peptide, CUB Domain, EGF Like 1 (SCUBE1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Signal Peptide, CUB Domain, EGF Like 1 (SCUBE1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Signal Peptide, CUB Domain, EGF Like 1 (SCUBE1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Signal Peptide, CUB Domain, EGF Like 1 (SCUBE1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 120587029
• NCBI GenBank Nucleotide #: NP_766638.2
• UniProt Accession #: Q8IWY4
• NCBI Official Full Name: signal peptide, CUB and EGF-like domain-containing protein 1
• UniProt Protein Name: Signal peptide, CUB and EGF-like domain-containing protein 1
• Protein Family: Signal peptide, CUB and EGF-like domain-containing protein
• UniProt Gene Name: SCUBE1
• UniProt Entry Name: SCUB1_HUMAN

Uniprot Description

SCUBE1: Could function as an adhesive molecule and its matrix bound and soluble fragments may play a critical role in vascular biology.

Chromosomal Location of Human Ortholog: 22q13

Cellular Component: extrinsic to plasma membrane; extracellular space; cell surface; external side of plasma membrane

Molecular Function: identical protein binding; protein heterodimerization activity; calcium ion binding

Biological Process: adult heart development; endothelial cell differentiation; blood coagulation; inflammatory response; protein homooligomerization; post-embryonic development