Rat SCUBE1 ELISA Kit | SCUBE1 elisa kit

Rat SCUBE1 (Signal Peptide, CUB and EGF-like Domain-containing Protein 1)ELISA Kit

Cat. Num. AAA2516219

• Reactivity: Rat
• Synonyms: SCUBE1; Rat SCUBE1 (Signal Peptide; CUB and EGF-like Domain-containing Protein 1)ELISA Kit; SCUBE1 elisa kit

• Reactivity: Rat
• Specificity: This kit recognizes natural and recombinantRatSCUBE1. No significant cross-reactivity or interference between RatSCUBE1 and analogues was observed.
• Sequence Length: 988

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.625-40ng/mL
• Sensitivity: Min: 0.375ng/mL; Max: 40ng/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SCUBE1 elisa kit

Intended Uses: This ELISA kit applies to the invitro quantitative determination of RatSCUBE1concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to SCUBE1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for SCUBE1and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain SCUBE1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of SCUBE1.You can calculate the concentration of SCUBE1in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 120587029
• NCBI GeneID: 80274
• NCBI Accession #: NP_766638.2
• NCBI GenBank Nucleotide #: NM_173050.3
• Molecular Weight: 107,910 Da
• NCBI Official Full Name: signal peptide, CUB and EGF-like domain-containing protein 1
• NCBI Official Synonym Full Names: signal peptide, CUB domain, EGF-like 1
• NCBI Official Symbol: SCUBE1
• NCBI Protein Information: signal peptide, CUB and EGF-like domain-containing protein 1; signal peptide-CUB domain-EGF-related 1
• UniProt Protein Name: Signal peptide, CUB and EGF-like domain-containing protein 1
• Protein Family: Signal peptide, CUB and EGF-like domain-containing protein
• UniProt Gene Name: SCUBE1
• UniProt Entry Name: SCUB1_HUMAN

NCBI Description

This gene encodes a cell surface glycoprotein that is a member of the SCUBE (signal peptide, CUB domain, EGF (epidermal growth factor)-like protein) family. Family members have an amino-terminal signal peptide, nine copies of EGF-like repeats and a CUB domain at the carboxyl terminus. This protein is expressed in platelets and endothelial cells and may play an important role in vascular biology. [provided by RefSeq, Oct 2011]

Uniprot Description

SCUBE1: Could function as an adhesive molecule and its matrix bound and soluble fragments may play a critical role in vascular biology.

Chromosomal Location of Human Ortholog: 22q13

Cellular Component: extrinsic to plasma membrane; extracellular space; cell surface; external side of plasma membrane

Molecular Function: identical protein binding; protein heterodimerization activity; calcium ion binding

Biological Process: adult heart development; endothelial cell differentiation; blood coagulation; inflammatory response; protein homooligomerization; post-embryonic development