Typical Testing Data/Standard Curve (for reference only)
Human signal peptide, CUB domain, EGF-like 1 ELISA Kit | SCUBE1 elisa kit
Human Signal Peptide, CUB and EGF-like Domain-containing Protein 1, SCUBE1 ELISA Kit
Reactivity
Human
Synonyms
signal peptide; CUB domain; EGF-like 1; Human Signal Peptide; CUB and EGF-like Domain-containing Protein 1; SCUBE1 ELISA Kit; CUB and EGF-like Domain-containing Protein 1 (SCUBE1) ELISA Kit; signal peptide-CUB domain-EGF-related 1; SCUBE1 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human SCUBE1. No significant cross-reactivity or interference between human SCUBE1 and analogues was observed.
Sequence Length
988
Samples
Serum, plasma, tissue homogenates.
Assay Type
Sandwich
Detection Range
0.625 ng/ml -40 ng/ml.
Sensitivity
0.156 ng /ml.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for SCUBE1 elisa kit
Principle of the Assay||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SCUBE1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SCUBE1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SCUBE1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SCUBE1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
107,910 Da
NCBI Official Full Name
signal peptide, CUB and EGF-like domain-containing protein 1
NCBI Official Synonym Full Names
signal peptide, CUB domain, EGF-like 1
NCBI Official Symbol
SCUBE1
NCBI Protein Information
signal peptide, CUB and EGF-like domain-containing protein 1; signal peptide-CUB domain-EGF-related 1
UniProt Protein Name
Signal peptide, CUB and EGF-like domain-containing protein 1
Protein Family
UniProt Gene Name
SCUBE1
UniProt Entry Name
SCUB1_HUMAN
NCBI Description
This gene encodes a cell surface glycoprotein that is a member of the SCUBE (signal peptide, CUB domain, EGF (epidermal growth factor)-like protein) family. Family members have an amino-terminal signal peptide, nine copies of EGF-like repeats and a CUB domain at the carboxyl terminus. This protein is expressed in platelets and endothelial cells and may play an important role in vascular biology. [provided by RefSeq, Oct 2011]
Uniprot Description
Function: Could function as an adhesive molecule and its matrix bound and soluble fragments may play a critical role in vascular biology.1 PublicationManual assertion based on experiment in:Ref.3
Research Articles on SCUBE1
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Product Notes
The Human SCUBE1 scube1 (Catalog #AAA705296) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA705296 ELISA Kit recognizes Human SCUBE1. It is sometimes possible for the material contained within the vial of "signal peptide, CUB domain, EGF-like 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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