Human SCUBE1 ELISA Kit | SCUBE1 elisa kit

Human SCUBE1 (Signal Peptide, CUB and EGF-like Domain-containing Protein 1)ELISA Kit

Cat. Num. AAA2515785

• Reactivity: Human
• Synonyms: SCUBE1; Human SCUBE1 (Signal Peptide; CUB and EGF-like Domain-containing Protein 1)ELISA Kit; SCUBE1 elisa kit

• Reactivity: Human
• Specificity: This kit recognizes Human SCUBE1 in samples. No significant cross-reactivity or interference between Human SCUBE1 and analogues was observed.
• Sequence Length: 988

• Samples: Serum, plasma and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.63-40ng/mL
• Sensitivity: 0.38ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human SCUBE1 were tested 20 times on one plate, respectively.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human SCUBE1 were tested on 3 different plates, 20 replicates in each plate.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SCUBE1 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human SCUBE1 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SCUBE1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SCUBE1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SCUBE1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human SCUBE1. You can calculate the concentration of Human SCUBE1 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 120587029
• NCBI GeneID: 80274
• NCBI Accession #: NP_766638.2
• NCBI GenBank Nucleotide #: NM_173050.3
• Molecular Weight: 107,910 Da
• NCBI Official Full Name: signal peptide, CUB and EGF-like domain-containing protein 1
• NCBI Official Synonym Full Names: signal peptide, CUB domain, EGF-like 1
• NCBI Official Symbol: SCUBE1
• NCBI Protein Information: signal peptide, CUB and EGF-like domain-containing protein 1; signal peptide-CUB domain-EGF-related 1
• UniProt Protein Name: Signal peptide, CUB and EGF-like domain-containing protein 1
• Protein Family: Signal peptide, CUB and EGF-like domain-containing protein
• UniProt Gene Name: SCUBE1
• UniProt Entry Name: SCUB1_HUMAN

NCBI Description

This gene encodes a cell surface glycoprotein that is a member of the SCUBE (signal peptide, CUB domain, EGF (epidermal growth factor)-like protein) family. Family members have an amino-terminal signal peptide, nine copies of EGF-like repeats and a CUB domain at the carboxyl terminus. This protein is expressed in platelets and endothelial cells and may play an important role in vascular biology. [provided by RefSeq, Oct 2011]

Uniprot Description

SCUBE1: Could function as an adhesive molecule and its matrix bound and soluble fragments may play a critical role in vascular biology.

Chromosomal Location of Human Ortholog: 22q13

Cellular Component: extrinsic to plasma membrane; extracellular space; cell surface; external side of plasma membrane

Molecular Function: identical protein binding; protein heterodimerization activity; calcium ion binding

Biological Process: adult heart development; endothelial cell differentiation; blood coagulation; inflammatory response; protein homooligomerization; post-embryonic development