Rabbit anti-Human Nanos1 Polyclonal Antibody | anti-NANOS1 antibody

Nanos1 Antibody

Cat. Num. AAA151552

• Gene Names: NANOS1; NOS1; SPGF12
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Nanos1 Antibody is affinity chromatography purified via peptide column.
• Synonyms: Nanos1; Polyclonal Antibody; Nanos1 Antibody; NOS1; SPGF12; Nanos homolog 1; NOS-1; nanos homolog 1 (Drosophila); anti-NANOS1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Nanos1 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 292

• Applicable Applications for anti-NANOS1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Nanos1 antibody can be used for detection of Nanos1 by Western blot at 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: Nanos1 antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human Nanos1.
• Buffer: Nanos1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: Nanos1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of Nanos1 in SK-N-SH cell lysate with Nanos1 antibody at 1 μg/mL in (A) the presence and (B) the absence of blocking peptide.)

Immunohistochemistry (IHC)

(Immunohistochemistry of Nanos1 in human brain tissue with Nanos1 antibody at 2.5 μg/mL)

Immunofluorescence (IF)

(Immunofluorescence of nanos1 in human brain tissue with nanos1 antibody at 20 μg/mL.)

Related Product Information for anti-NANOS1 antibody

Nanos1 Antibody: Nanos1 is one of three known mammalian homologs to the Drosophila gene nanos. Nanos1 is an RNA-binding protein containing a zinc-finger motif and is expressed in the developing nervous system and continues in the adult brain. Interestingly, unlike mice deficient in either nanos2 or nanos3, mice lacking the nanos1 gene develop normally with no sign of abnormalities. Recently it has been found that expression of nanos1 mRNA is down-regulated by E-cadherin in a human breast cancer cell line and the amino-terminal domain on Nanos1 interacts with the E-cadherin-binding protein p120ctn. Furthermore, overexpression of Nanos1 in human colorectal DLD1 cancer cells functionally abolished cell-cell adhesion, allowing the cancer cells to develop strong migratory and invasive properties. These results suggest that targeting Nanos1 might prove an effective strategy in the treatment of E-cadherin-negative tumors.

NCBI and Uniprot Product Information

• NCBI GI #: 41688589
• NCBI GeneID: 340719
• UniProt Accession #: Q8WY41
• Molecular Weight: 30,230 Da
• NCBI Official Full Name: Nanos homolog 1
• NCBI Official Synonym Full Names: nanos homolog 1 (Drosophila)
• NCBI Official Symbol: NANOS1
• NCBI Official Synonym Symbols: NOS1; SPGF12
• NCBI Protein Information: nanos homolog 1; NOS-1; EC_Rep1a
• UniProt Protein Name: Nanos homolog 1
• Protein Family: Nanos
• UniProt Gene Name: NANOS1
• UniProt Synonym Gene Names: NOS1; NOS-1
• UniProt Entry Name: NANO1_HUMAN

NCBI Description

This gene encodes a CCHC-type zinc finger protein that is a member of the nanos family. This protein co-localizes with the RNA-binding protein pumilio RNA-binding family member 2 and may be involved in regulating translation as a post-transcriptional repressor. Mutations in this gene are associated with spermatogenic impairment. [provided by RefSeq, Sep 2015]

Uniprot Description

NANOS1: May act as a translational repressor which regulates translation of specific mRNAs by forming a complex with PUM2 that associates with the 3'-UTR of mRNA targets. Capable of interfering with the proadhesive and anti-invasive functions of E-cadherin. Up-regulates the production of MMP14 to promote tumor cell invasion. Belongs to the nanos family.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 10q26.11

Cellular Component: perinuclear region of cytoplasm; cytoplasm

Molecular Function: protein binding; translation repressor activity; zinc ion binding; RNA binding

Biological Process: cell migration; negative regulation of translation

Disease: Spermatogenic Failure 12