Western Blot (WB)
(Western blot analysis of caspase-13 in human HL60 cell lysate (A), mouse brain (B) and rat brain (C) tissue lysates with caspase-13 antibody at 1 μg/mL.)
Rabbit Caspase-13 Polyclonal Antibody | anti-CASP4 antibody
Caspase-13 Antibody
Gene Names
CASP4; CASP13
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Caspase-13 Antibody is Ion exchange chromatography purified.
Synonyms
Caspase-13; Polyclonal Antibody; Caspase-13 Antibody; CASP13; Evolutionary related interleukin-1-beta-converting enzyme; CASP-13; caspase 4; apoptosis-related cysteine peptidase; anti-CASP4 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Caspase-13 Antibody is Ion exchange chromatography purified.
Form/Format
Liquid
Sequence Length
377
Applicable Applications for anti-CASP4 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Caspase-13 antibody can be used for detection of caspase-13 by Western blot at 1 mug/mL. A 43 kDa band should be detected. Antibody can also be used for immunohistochemistry starting at 10 mug/mL.
Conjugate
Unconjugated
Immunogen
Caspase-13 antibody was raised against a 15 amino acid peptide near the amino terminus of human Caspase-13.
Buffer
Caspase-13 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
Caspase-13 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Western Blot (WB)
(Western blot analysis of caspase-13 in human HL60 cell lysate (A), mouse brain (B) and rat brain (C) tissue lysates with caspase-13 antibody at 1 μg/mL.)
Western Blot (WB)
(Western blot analysis of caspase-13 in human HL60 cell lysate (A), mouse brain (B) and rat brain (C) tissue lysates with caspase-13 antibody at 1 μg/mL.)
Immunohistochemistry (IHC)
(Immunohistochemistry of caspase-13 in mouse brain tissue with caspase-13 antibody at 10 μg/mL.)
Immunohistochemistry (IHC)
(Immunohistochemistry of caspase-13 in mouse brain tissue with caspase-13 antibody at 10 μg/mL.)
Related Product Information for anti-CASP4 antibody
Caspase-13 Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death is finally caused by members of the caspase family of proteases and caspase activated DNases. A novel member in the caspase family was recently identified and designated ERICE (for Evolutionarily Related Interleukin-1 beta Converting Enzyme) and caspase-13. Caspase-13 belongs to the ICE subfamily of caspases. Overexpression of caspase-13 induces apoptosis. Caspase-13 was activated by caspase-8, which is a key enzyme in death receptor induced apoptosis. Caspase-13 is expressed in a variety of human tissues and cell lines.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
43 kDa
NCBI Official Full Name
Caspase-13
NCBI Official Synonym Full Names
caspase 4, apoptosis-related cysteine peptidase
NCBI Official Symbol
CASP4
NCBI Official Synonym Symbols
CASP13
NCBI Protein Information
caspase-4; ERICE; CASP-4; CASP-13; caspase 13; caspase-13; caspase 4, apoptosis-related cysteine protease; evolutionary related interleukin-1-beta-converting enzyme
UniProt Protein Name
Caspase-13
Protein Family
UniProt Gene Name
CASP13
UniProt Synonym Gene Names
CASP-13; ERICE
UniProt Entry Name
CASPD_BOVIN
Uniprot Description
CASP4: Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves caspase-1. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a small and a large subunit. Widely expressed, with highest levels in spleen and lung. Moderate expression in heart and liver, low expression in skeletal muscle, kidney and testis. Not found in the brain. Belongs to the peptidase C14A family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.4.22.64; Apoptosis; Protease
Cellular Component: endoplasmic reticulum membrane; mitochondrion; cytoplasm
Molecular Function: cysteine-type endopeptidase activity
Biological Process: regulation of apoptosis; apoptosis; regulation of inflammatory response; germ cell programmed cell death; positive regulation of interleukin-1 beta secretion; proteolysis
Similar Products
Product Notes
The CASP4 casp13 (Catalog #AAA151066) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Caspase-13 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Caspase-13 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Caspase-13 antibody can be used for detection of caspase-13 by Western blot at 1 mug/mL. A 43 kDa band should be detected. Antibody can also be used for immunohistochemistry starting at 10 mug/mL. Researchers should empirically determine the suitability of the CASP4 casp13 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Caspase-13, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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