Rabbit AIF Polyclonal Antibody | anti-CNKSR1 antibody

AIF Antibody

Cat. Num. AAA150788

• Gene Names: CNKSR1; CNK; CNK1
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: AIF Antibody is Ion exchange chromatography purified.
• Synonyms: AIF; Polyclonal Antibody; AIF Antibody; CNK; KSR; CNK1; Connector enhancer of kinase suppressor of ras 1; CNK homolog protein 1; Connector enhancer of KSR 1; connector enhancer of kinase suppressor of Ras 1; anti-CNKSR1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: AIF Antibody is Ion exchange chromatography purified.
• Form/Format: Liquid
• Sequence Length: 720

• Applicable Applications for anti-CNKSR1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: AIF antibody can be used for detection of AIF by Western blot at 1 mug/mL. A 67 kDa band should be detected. Antibody can also be used for immunohistochemistry starting at 10 mug/mL.
• Conjugate: Unconjugated
• Immunogen: AIF antibody was raised against a 15 amino acid peptide near the center of human AIF.
• Buffer: AIF Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: AIF antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of AIF in K562 cell lysate (A), rat heart (B), and mouse heart (C) tissue lysates with AIF antibody (IN) at 1 μg/mL. )

Immunohistochemistry (IHC)

(Immunohistochemistry of AIF in human retina tissue with AIF antibody at 10 μg/mL.)

Related Product Information for anti-CNKSR1 antibody

AIF Antibody: Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins. A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF). Like the critical molecules, cytochrome c and caspase-9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and large scale DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection and apoptosis stimuli. AIF is highly conserved between human and mouse and widely expressed.

NCBI and Uniprot Product Information

• NCBI GI #: 50400606
• NCBI GeneID: 10256
• UniProt Accession #: O95381; B1AMW9; Q969H4
• Molecular Weight: 67 kDa
• NCBI Official Full Name: Connector enhancer of kinase suppressor of ras 1
• NCBI Official Synonym Full Names: connector enhancer of kinase suppressor of Ras 1
• NCBI Official Symbol: CNKSR1
• NCBI Official Synonym Symbols: CNK; CNK1
• NCBI Protein Information: connector enhancer of kinase suppressor of ras 1; CNK homolog protein 1; connector enhancer of KSR 1
• UniProt Protein Name: Connector enhancer of kinase suppressor of ras 1
• Protein Family: Connector enhancer of kinase suppressor of ras
• UniProt Gene Name: CNKSR1
• UniProt Synonym Gene Names: CNK1; Connector enhancer of KSR 1; CNK1; hCNK1
• UniProt Entry Name: CNKR1_HUMAN

NCBI Description

This gene encodes a protein containing several motifs involved in protein-protein interaction, including PDZ, PH (Pleckstrin homology), and SAM (sterile alpha motif) domains. The encoded protein acts as a scaffold component for receptor tyrosine kinase signaling and may mediate crosstalk between different signaling pathways. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Uniprot Description

CNK1: a RAF-binding multidomain protein required for RAS signaling. Contains several protein-protein interaction domains. Its PH domain interacts with Rho in a GTP-dependent manner. Associates with Rhophilin and RalGDS, Rho and Ras effector molecules. Two alternatively spliced isoforms have been described.

Protein type: G protein regulator, misc.; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 1p36.11

Cellular Component: plasma membrane; cell cortex; intercellular junction

Molecular Function: protein binding, bridging

Biological Process: Ras protein signal transduction; transmembrane receptor protein tyrosine kinase signaling pathway; Rho protein signal transduction