Rabbit anti-Human Caspase-6 Polyclonal Antibody | anti-CASP6 antibody

Caspase-6 Antibody

Cat. Num. AAA151565

• Gene Names: CASP6; MCH2
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: Caspase-6 Antibody is immunoaffinity purified.
• Synonyms: Caspase-6; Polyclonal Antibody; Caspase-6 Antibody; MCH2; Apoptotic protease Mch-2; CASP-6; caspase 6; apoptosis-related cysteine peptidase; anti-CASP6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Depending on cell lines or tissues used, either full-length or other cleavage products may be observed.
• Purity/Purification: Caspase-6 Antibody is immunoaffinity purified.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 293

• Applicable Applications for anti-CASP6 antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: Casp-6 antibody can be used for the detection of Caspase-6 by Western blot at 1 and 2 mug/mL. Antibody can also be used for immunocytochemistry starting at 1 mug/mL. For immunofluorescence start at 10 mug/mL.
• Conjugate: Unconjugated
• Immunogen: Caspase-6 antibody was raised against a 15 amino acid synthetic peptide from near the center of human Caspase-6.
• Buffer: Caspase-6 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: Caspase-6 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of Caspase-6 in MCF7 cell lysate with Caspase-6 antibody (IN) at (A) 1 and (B) 2 μg/mL. )

Immunocytochemistry (IHC)

(Immunocytochemistry of caspase-6 in MCF7 with caspase-6 antibody at 1 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of Caspase 6 in MCF7 cells with Caspase 6 antibody at 10 μg/mL.)

Related Product Information for anti-CASP6 antibody

Caspase-6 Antibody: Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. The apoptotic subfamily can be further divided into initiator caspases, which are activated in response to death signals, and executioner caspases, which are activated by the initiator caspases and are responsible for cleavage of cellular substrates that ultimately lead to cell death. Caspase-6 is an executioner caspase that was identified based on its homology with human caspases 2 and 3 as well as the C. elegans cell death protein CED-3. It possesses two isoforms, of which only the longer form possesses protease activity. Caspase-6 is highly expressed in adult brain and may play a role in several neuronal pathologies.

NCBI and Uniprot Product Information

• NCBI GI #: 14916483
• NCBI GeneID: 839
• NCBI Accession #: NP_001217
• NCBI GenBank Nucleotide #: NM_001226.3
• UniProt Accession #: P55212; Q9BQE7
• Molecular Weight: 22,574 Da
• NCBI Official Full Name: caspase-6 isoform alpha preproprotein
• NCBI Official Synonym Full Names: caspase 6, apoptosis-related cysteine peptidase
• NCBI Official Symbol: CASP6
• NCBI Official Synonym Symbols: MCH2
• NCBI Protein Information: caspase-6; apoptotic protease MCH-2; caspase 6, apoptosis-related cysteine protease
• UniProt Protein Name: Caspase-6
• Protein Family: Caspase
• UniProt Gene Name: CASP6
• UniProt Synonym Gene Names: MCH2; CASP-6
• UniProt Entry Name: CASP6_HUMAN

NCBI Description

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspases 7, 8 and 10, and is thought to function as a downstream enzyme in the caspase activation cascade. Alternative splicing of this gene results in two transcript variants that encode different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

CASP6: Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 18 kDa (p18) and a 11 kDa (p11) subunit. Interacts with BIRC6/bruce. Activation is suppressed by phosphorylation at Ser-257. Belongs to the peptidase C14A family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; EC 3.4.22.59; Protease

Chromosomal Location of Human Ortholog: 4q25

Cellular Component: nucleoplasm; cytoplasm; cytosol

Molecular Function: identical protein binding; protein binding; cysteine-type endopeptidase activity; cysteine-type peptidase activity

Biological Process: epithelial cell differentiation; apoptosis; proteolysis; cell structure disassembly during apoptosis