Rabbit anti-Human Tumor necrosis factor receptor type 1-associated DEATH domain protein Polyclonal Antibody | anti-TRADD antibody

Rabbit anti-human Tumor necrosis factor receptor type 1-associated DEATH domain protein polyclonal Antibody(TRADD)

Cat. Num. AAA1495986

• Gene Names: TRADD; Hs.89862
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Antigen Affinity Purified
• Synonyms: Tumor necrosis factor receptor type 1-associated DEATH domain protein; Polyclonal Antibody; Rabbit anti-human Tumor necrosis factor receptor type 1-associated DEATH domain protein polyclonal Antibody(TRADD); TNFR1-associated DEATH domain protein; TNFRSF1A-associated via death domain; TRADD; anti-TRADD antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purified
• Sequence Length: 312

• Applicable Applications for anti-TRADD antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Recommended dilution: WB:1:500-2000; IHC:1:20-1:200
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Conjugate: Non-conjugated
• Immunogen: Recombinant human Tumor necrosis factor receptor type 1-associated DEATH domain protein (1-312aa)
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-1163 / sc-7868
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(All lanes: Tumor necrosis factor receptor type 1-associated DEATH domain protein antibody at 12ug/ml+ MCF-7 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 35,28 kDaObserved band size: 34 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human endometrial cancer using MBS1495986 at dilution 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human colon cancer using MBS1495986 at dilution 1:100)

Related Product Information for anti-TRADD antibody

The nuclear form acts as a tumor suppressor by preventing ubiquitination and degradation of isoform p19ARF/ARF of CDKN2A by TRIP12: acts by interacting with TRIP12, leading to disrupt interaction between TRIP12 and isoform p19ARF/ARF of CDKN2A (By similarity). Adapter molecule for TNFRSF1A/TNFR1 that specifically associates with the cytoplasmic domain of activated TNFRSF1A/TNFR1 mediating its interaction with FADD. Overexpression of TRADD leads to two major TNF-induced responses, apoptosis and activation of NF-kappa-B.

NCBI and Uniprot Product Information

• NCBI GI #: 13378137
• NCBI GeneID: 8717
• NCBI Accession #: NP_003780.1
• NCBI GenBank Nucleotide #: NM_003789.3
• UniProt Accession #: Q15628; Q52NZ1; B2RDS3; B3KQZ9
• Molecular Weight: 27,904 Da
• NCBI Official Full Name: tumor necrosis factor receptor type 1-associated DEATH domain protein
• NCBI Official Synonym Full Names: TNFRSF1A-associated via death domain
• NCBI Official Symbol: TRADD
• NCBI Official Synonym Symbols: Hs.89862
• NCBI Protein Information: tumor necrosis factor receptor type 1-associated DEATH domain protein
• UniProt Protein Name: Tumor necrosis factor receptor type 1-associated DEATH domain protein
• Protein Family: Tumor necrosis factor receptor type 1-associated DEATH domain protein
• UniProt Gene Name: TRADD
• UniProt Synonym Gene Names: TNFR1-associated DEATH domain protein
• UniProt Entry Name: TRADD_HUMAN

NCBI Description

The protein encoded by this gene is a death domain containing adaptor molecule that interacts with TNFRSF1A/TNFR1 and mediates programmed cell death signaling and NF-kappaB activation. This protein binds adaptor protein TRAF2, reduces the recruitment of inhibitor-of-apoptosis proteins (IAPs) by TRAF2, and thus suppresses TRAF2 mediated apoptosis. This protein can also interact with receptor TNFRSF6/FAS and adaptor protein FADD/MORT1, and is involved in the Fas-induced cell death pathway. [provided by RefSeq, Jul 2008]

Uniprot Description

TRADD: Adapter molecule for TNFRSF1A/TNFR1 that specifically associates with the cytoplasmic domain of activated TNFRSF1A/TNFR1 mediating its interaction with FADD. Overexpression of TRADD leads to two major TNF-induced responses, apoptosis and activation of NF-kappa-B. Heterodimer with TNFRSF1A/TNFR1. Interacts with DAB2IP, FADD, HIPK2, KRT14, KRT16, KRT17, KRT18, RIPK1, SQSTM1, TRAF1, TRAF2 and TRPC4AP. Found in all examined tissues.

Protein type: Adaptor/scaffold; Apoptosis

Chromosomal Location of Human Ortholog: 16q22

Cellular Component: cytoplasm; cytoskeleton; cytosol; lipid raft; nucleus; plasma membrane; receptor complex

Molecular Function: identical protein binding; kinase binding; molecular adaptor activity; protein binding; protein complex binding; signal transducer activity; tumor necrosis factor receptor binding

Biological Process: activation of NF-kappaB transcription factor; apoptosis; caspase activation; cell surface receptor linked signal transduction; I-kappaB kinase/NF-kappaB cascade; induction of apoptosis via death domain receptors; negative regulation of caspase activity; positive regulation of apoptosis; positive regulation of hair follicle development; positive regulation of I-kappaB kinase/NF-kappaB cascade; programmed cell death; protein heterooligomerization; signal transduction; tumor necrosis factor-mediated signaling pathway