Rabbit BRSK1 Polyclonal Antibody | anti-BRSK1 antibody

BRSK1 Antibody

Cat. Num. AAA150240

• Gene Names: BRSK1; hSAD1
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: BRSK1 Antibody is affinity chromatography purified via peptide column.
• Synonyms: BRSK1; Polyclonal Antibody; BRSK1 Antibody; hSAD1; KIAA1811; SAD1; SADB; Brain-selective kinase 1; SAD1 homolog; BR serine/threonine kinase 1; anti-BRSK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: BRSK1 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 794

• Applicable Applications for anti-BRSK1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: BRSK1 antibody can be used for detection of BRSK1 by Western blot at 0.5 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: BRSK1 antibody was raised against a 28 amino acid synthetic peptide from near the center of human BRSK1.
• Buffer: BRSK1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: BRSK1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of BRSK1 in human brain tissue lysate with BRSK1 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of BRSK1 in human brain tissue with BRSK1 antibody at 5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of BRSK1 in Human Brain cells with BRSK1 antibody at 20 μg/mL.)

Related Product Information for anti-BRSK1 antibody

BRSK1 Antibody: BRSK1 was initially identified as a mammalian homolog to the fission yeast S. pombe Cdr2, a mitosis-regulatory kinase and also shows significant homology to the C. elegans neuronal cell polarity regulator SAD1. BRSK1 is unbiquitously expressed, with highest levels of expression in the brain and testes. Similar to its yeast homolog, BRSK1 is thought to be involved in stress-induced cell cycle arrest. Overexpression of this protein leads to the G2/M arrest in HeLa S2 cells and UV-induced G2/M arrest could be partially abrogated by reduced expression of BRSK1 through the use of siRNA, indicating its role in DNA damage checkpoint function. More recently, it has been shown that both BRSK1 and the related protein BRSK2 are required for mammalian neuronal polarization. While BRSK1- and BRSK2-null mice were viable, double-mutant mice died within two hours of birth. Neurons from these mice showed uniformly-sized neurites as opposed to the normal long axon and multiple shorter dendrites. These neurites also displayed both axonal and dendritic markers. At least two isoforms of BRSK1 are known to exist.

NCBI and Uniprot Product Information

• NCBI GI #: 34395684
• NCBI GeneID: 84446
• UniProt Accession #: Q8TDC3; Q5J5B5; Q8NDD0; Q8NDR4; Q8TDC2; Q96AV4; Q96JL4; F1DG44
• Molecular Weight: 38,782 Da
• NCBI Official Full Name: BR serine/threonine-protein kinase 1
• NCBI Official Synonym Full Names: BR serine/threonine kinase 1
• NCBI Official Symbol: BRSK1
• NCBI Official Synonym Symbols: hSAD1
• NCBI Protein Information: serine/threonine-protein kinase BRSK1; SAD1 kinase; SAD1 homolog; protein kinase SAD1A; brain-selective kinase 1; SadB kinase short isoform; BR serine/threonine-protein kinase 1; serine/threonine-protein kinase SAD-B; synapses of Amphids Defective homolog 1; brain-specific serine/threonine-protein kinase 1
• UniProt Protein Name: Serine/threonine-protein kinase BRSK1
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: BRSK1
• UniProt Synonym Gene Names: KIAA1811; SAD1; SADB; BR serine/threonine-protein kinase 1; SAD1 homolog; hSAD1
• UniProt Entry Name: BRSK1_HUMAN

Uniprot Description

BRSK1: a serine/threonine protein kinase of the CAMK group. Closely related to AMPK. Activated by the kinase LKB1. Acts as checkpoint kinase upon DNA damage induced by UV or methyl methane sulfonate. Phosphorylates WEE1 and CDC25B in vitro and CDC25C in vitro and in vivo. Translocates from the cytoplasm to the nucleus in response to UV- or MMS-induced DNA damage. BRSK1 and BRSK2 are required for neuronal polarization. Widely expressed with highest levels in the brain and testis. Two alternatively spliced isoforms have been described.

Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; EC 2.7.11.26; Kinase, protein; EC 2.7.11.1; CAMK group; CAMKL family; BRSK subfamily

Chromosomal Location of Human Ortholog: 19q13.4

Cellular Component: nucleoplasm; centrosome; synaptic vesicle; cytoplasm; nucleus; cell junction

Molecular Function: protein serine/threonine kinase activity; gamma-tubulin binding; tau-protein kinase activity; magnesium ion binding; protein kinase binding; ATP binding

Biological Process: neuron differentiation; axonogenesis; establishment of cell polarity; neurotransmitter secretion; response to ionizing radiation; G2/M transition DNA damage checkpoint; protein amino acid phosphorylation; response to DNA damage stimulus; centrosome duplication; response to UV