Human ADAM metallopeptidase with ombospondin type 1 motif ELISA Kit | ADAMTS13 elisa kit

Human ADAM metallopeptidase with ombospondin type 1 motif, 13 (ADAMTS13) ELISA Kit

Cat. Num. AAA2606935

• Reactivity: Human
• Synonyms: ADAM metallopeptidase with ombospondin type 1 motif; Human ADAM metallopeptidase with ombospondin type 1 motif; 13 (ADAMTS13) ELISA Kit; ADAMTS13 elisa kit

• Reactivity: Human
• Sequence Length: 1935

• Samples: Human serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 50 ng/mL-0.78 ng/mL
• Sensitivity: Up to 0.5 ng/mL
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ADAMTS13 elisa kit

Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Human ADAMTS13 monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 120659948
• NCBI GeneID: 56999
• Molecular Weight: 213,420 Da
• NCBI Official Full Name: ADAM metallopeptidase with thrombospondin type 1 motif, 9
• NCBI Official Synonym Full Names: ADAM metallopeptidase with thrombospondin type 1 motif 9
• NCBI Official Symbol: ADAMTS9
• NCBI Protein Information: A disintegrin and metalloproteinase with thrombospondin motifs 9
• UniProt Protein Name: A disintegrin and metalloproteinase with thrombospondin motifs 9
• Protein Family: A disintegrin and metalloproteinase with thrombospondin motifs
• UniProt Gene Name: ADAMTS9
• UniProt Synonym Gene Names: KIAA1312; ADAM-TS 9; ADAM-TS9; ADAMTS-9
• UniProt Entry Name: ATS9_HUMAN

NCBI Description

This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. Members of the ADAMTS family have been implicated in the cleavage of proteoglycans, the control of organ shape during development, and the inhibition of angiogenesis. This gene is localized to chromosome 3p14.3-p14.2, an area known to be lost in hereditary renal tumors. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing. [provided by RefSeq, Jan 2016]

Uniprot Description

ADAMTS9: Cleaves the large aggregating proteoglycans, aggrecan and versican. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; EC 3.4.24.-; Extracellular matrix; Motility/polarity/chemotaxis; Protease; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3p14.1

Cellular Component: endoplasmic reticulum; extracellular matrix; proteinaceous extracellular matrix

Molecular Function: metalloendopeptidase activity; metallopeptidase activity; zinc ion binding

Biological Process: glycoprotein catabolic process; multicellular organismal development; protein transport; proteolysis; vesicle-mediated transport