Rabbit anti-Mouse Bis (5-adenosyl)-triphosphatase Polyclonal Antibody | anti-Fhit antibody

Rabbit anti-mouse Bis(5-adenosyl)-triphosphatase polyclonal Antibody, FITC

Cat. Num. AAA1491704

• Gene Names: Fhit; Fra14A2; AW045638
• Reactivity: Mouse
• Applications: ELISA
• Purity: Caprylic Acid Ammonium Sulfate Precipitation Purified
• Synonyms: Bis (5-adenosyl)-triphosphatase; Polyclonal Antibody; Rabbit anti-mouse Bis(5-adenosyl)-triphosphatase polyclonal Antibody; FITC; AP3A hydrolase; Diadenosine 5; 5-P1; P3-triphosphate hydrolase; Dinucleosidetriphosphatase; Fragile histidine triad protein; Fhit; anti-Fhit antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Caprylic Acid Ammonium Sulfate Precipitation Purified
• Sequence Length: 150

• Applicable Applications for anti-Fhit antibody: ELISA (EIA)
• Storage Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Conjugate: FITC
• Immunogen: Recombinant mouse Bis(5-adenosyl)-triphosphatase protein(2-150AA)
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Related Product Information for anti-Fhit antibody

Cleaves P(1)-P(3)-bis(5'-adenosyl) triphosphate (Ap3A) to yield AMP and ADP. Can also hydrolyze P(1)-P(4)-bis(5'-adenosyl) tetraphosphate (Ap4A), but has extremely low activity with ATP. Modulates transcriptional activation by CTNNB1 and thereby contributes to regulate the expression of genes essential for cell proliferation and survival, such as CCND1 and BIRC5. Plays a role in the induction of apoptosis via SRC and AKT1 signaling pathways. Inhibits MDM2-mediated proteasomal degradation of p53/TP53 and thereby plays a role in p53/TP53-mediated apoptosis. Induction of apoptosis depends on the ability of FHIT to bind P(1)-P(3)-bis(5'-adenosyl) triphosphate or related compounds, but does not require its catalytic activity (By similarity). Functions as tumor suppressor.

NCBI and Uniprot Product Information

• NCBI GI #: 815930058
• NCBI GeneID: 14198
• NCBI Accession #: NP_001295215.1
• NCBI GenBank Nucleotide #: NM_001308286.1
• UniProt Accession #: O89106; Q6URW5; Q91VL1
• Molecular Weight: 17,235 Da
• NCBI Official Full Name: bis(5'-adenosyl)-triphosphatase isoform 2
• NCBI Official Synonym Full Names: fragile histidine triad gene
• NCBI Official Symbol: Fhit
• NCBI Official Synonym Symbols: Fra14A2; AW045638
• NCBI Protein Information: bis(5'-adenosyl)-triphosphatase
• UniProt Protein Name: Bis(5'-adenosyl)-triphosphatase
• Protein Family: Bis(5'-adenosyl)-triphosphatase
• UniProt Gene Name: Fhit
• UniProt Synonym Gene Names: AP3Aase
• UniProt Entry Name: FHIT_MOUSE

NCBI Description

This gene encodes a member of the HIT family of proteins that are characterized by the presence of a histidine triad sequence. The encoded protein is a diadenosine triphosphate hydrolase enzyme that cleaves the P(1)-P(3)-bis(5'-adenosyl) triphosphate (Ap3A) to yield AMP and ADP. This locus is very fragile and has been found to be altered in different types of cancers. Mice lacking the encoded protein display increased susceptibility to spontaneous and induced tumors. Ectopic expression of the encoded protein in such knockout mice inhibits tumor development. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]

Uniprot Description

FHIT: a member of the histidine triad gene family. A diadenosine 5',5'''-P1,P3-triphosphate hydrolase involved in purine metabolism. Its gene encompasses the common fragile site FRA3B on chromosome 3, where carcinogen-induced damage can lead to translocations and aberrant transcripts of this gene. A possible tumor suppressor in specific tissues. Phospho-FHIT observed in liver and kidney, but not in brain and lung. Phospho-FHIT undetected in all tested human tumor cell lines. Aberrant transcripts from this gene have been found in about half of all esophageal, stomach, and colon carcinomas.

Protein type: Motility/polarity/chemotaxis; EC 3.6.1.29; DNA replication; Nucleotide Metabolism - purine; Hydrolase; Tumor suppressor

Cellular Component: cytoplasm; cytosol; intracellular; nucleus; plasma membrane

Molecular Function: bis(5'-adenosyl)-triphosphatase activity; catalytic activity; hydrolase activity; identical protein binding; nickel ion binding; nucleotide binding; ubiquitin protein ligase binding

Biological Process: apoptosis; diadenosine triphosphate catabolic process; DNA replication; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; nucleotide metabolic process; purine nucleotide metabolic process; regulation of transcription, DNA-dependent; transcription, DNA-dependent