Rabbit anti-Human FHIT Polyclonal Antibody | anti-FHIT antibody

Anti-FHIT Antibody (aa80-129)

Cat. Num. AAA2401483

• Gene Names: FHIT; FRA3B; AP3Aase
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry
• Purity: Immunoaffinity Purified
• Synonyms: FHIT; Polyclonal Antibody; Anti-FHIT Antibody (aa80-129); Rabbit Polyclonal (IgG) to Human FHIT; AP3Aase; Dinucleosidetriphosphatase; Fragile histidine triad; Fragile histidine triad gene; AP3A hydrolase; Bis(5-adenosyl)-triphosphatase; FRA3B; Tumor suppressor protein; anti-FHIT antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: FHIT Antibody detects endogenous levels of FHIT.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol.
• Sequence Length: 147

• Applicable Applications for anti-FHIT antibody: ELISA (EIA), Immunohistochemistry (IHC) Paraffin
• Application Notes: ELISA: 1:10000; IHC-P: 1:33-1:50
• Immunogen: Synthetic peptide derived from human FHIT (aa80-129).
• Conjugation: Unconjugated
• Epitope: aa80-129
• Preparation and Storage: Store at -20 degree C.

Immunohistochemistry (IHC)

(Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE))

Related Product Information for anti-FHIT antibody

Cleaves P(1)-P(3)-bis(5'-adenosyl) triphosphate (Ap3A) to yield AMP and ADP. Can also hydrolyze P(1)-P(4)-bis(5'-adenosyl) tetraphosphate(Ap4A), but has extremely low activity with ATP. Modulatestranscriptional activation by CTNNB1 and thereby contributes to regulatethe expression of genes essential for cell proliferation and survival, such as CCND1 and BIRC5. Plays a role in the induction of apoptosis viaSRC and AKT1 signaling pathways.

NCBI and Uniprot Product Information

• NCBI GI #: 4503719
• NCBI GeneID: 2272
• NCBI Accession #: NP_002003.1
• NCBI GenBank Nucleotide #: NM_002012.3
• UniProt Accession #: P49789; Q45QG9; Q6IU12; A2IAS9; A2IAT0; A2IAT6; A8K1A9
• Molecular Weight: 16,858 Da
• NCBI Official Full Name: bis(5'-adenosyl)-triphosphatase isoform 1
• NCBI Official Synonym Full Names: fragile histidine triad
• NCBI Official Symbol: FHIT
• NCBI Official Synonym Symbols: FRA3B; AP3Aase
• NCBI Protein Information: bis(5'-adenosyl)-triphosphatase
• UniProt Protein Name: Bis(5'-adenosyl)-triphosphatase
• Protein Family: Bis(5'-adenosyl)-triphosphatase
• UniProt Gene Name: FHIT
• UniProt Synonym Gene Names: AP3Aase

NCBI Description

The protein encoded by this gene is a P1-P3-bis(5'-adenosyl) triphosphate hydrolase involved in purine metabolism. This gene encompasses the common fragile site FRA3B on chromosome 3, where carcinogen-induced damage can lead to translocations and aberrant transcripts. In fact, aberrant transcripts from this gene have been found in about half of all esophageal, stomach, and colon carcinomas. The encoded protein is also a tumor suppressor, as loss of its activity results in replication stress and DNA damage. [provided by RefSeq, Aug 2017]

Uniprot Description

Cleaves P1-P3-bis(5'-adenosyl) triphosphate (Ap3A) to yield AMP and ADP. Can also hydrolyze P1-P4-bis(5'-adenosyl) tetraphosphate (Ap4A), but has extremely low activity with ATP. Modulates transcriptional activation by CTNNB1 and thereby contributes to regulate the expression of genes essential for cell proliferation and survival, such as CCND1 and BIRC5. Plays a role in the induction of apoptosis via SRC and AKT1 signaling pathways. Inhibits MDM2-mediated proteasomal degradation of p53/TP53 and thereby plays a role in p53/TP53-mediated apoptosis. Induction of apoptosis depends on the ability of FHIT to bind P1-P3-bis(5'-adenosyl) triphosphate or related compounds, but does not require its catalytic activity, it may in part come from the mitochondrial form, which sensitizes the low-affinity Ca2+ transporters, enhancing mitochondrial calcium uptake. Functions as tumor suppressor.