Rabbit anti-Human Bis(5'-adenosyl)-triphosphatase Polyclonal Antibody | anti-FHIT antibody

Bis(5'-adenosyl)-triphosphatase Polyclonal Antibody

Cat. Num. AAA9416456

• Gene Names: FHIT; FRA3B; AP3Aase
• Reactivity: Human
• Applications: Western Blot
• Purity: Caprylic Acid Ammonium Sulfate Precipitation purified
• Synonyms: Bis(5'-adenosyl)-triphosphatase; Polyclonal Antibody; Bis(5'-adenosyl)-triphosphatase Polyclonal Antibody; AP3A hydrolase; AP3Aase; Diadenosine 5'; 5'''-P1; P3-triphosphate hydrolase; Dinucleosidetriphosphatase; Fragile histidine triad protein; anti-FHIT antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total Bis(5'-adenosyl)-triphosphatase polyclonal antibody.
• Purity/Purification: Caprylic Acid Ammonium Sulfate Precipitation purified
• Form/Format: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 147

• Applicable Applications for anti-FHIT antibody: Western Blot (WB)
• Application Notes: Western Blot: 1:500 - 1:1000
• Immunogen: Recombinant Human Bis(5'-adenosyl)-triphosphatase
• Immunogen Type: Protein
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(All lanes: Bis(5'-adenosyl)-triphosphatase antibody at 2ug/ml Lane 1 : EC109 whole cell lysate Lane 2 : 293T whole cell lysat)

Related Product Information for anti-FHIT antibody

Cleaves P(1)-P(3)-bis(5'-adenosyl) triphosphate (Ap3A) to yield AMP and ADP. Can also hydrolyze P(1)-P(4)-bis(5'-adenosyl) tetraphosphate (Ap4A), but has extremely low activity with ATP. Modulates transcriptional activation by CTNNB1 and thereby contributes to regulate the expression of genes essential for cell proliferation and survival, such as CCND1 and BIRC5. Plays a role in the induction of apoptosis via SRC and AKT1 signaling pathways. Inhibits MDM2-mediated proteasomal degradation of p53/TP53 and thereby plays a role in p53/TP53-mediated apoptosis. Induction of apoptosis depends on the ability of FHIT to bind P(1)-P(3)-bis(5'-adenosyl) triphosphate or related compounds, but does not require its catalytic activity, it may in part come from the mitochondrial form, which sensitizes the low-affinity Ca2+ transporters, enhancing mitochondrial calcium uptake. Functions as tumor suppressor.

Product Categories/Family for anti-FHIT antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 261278358
• NCBI GeneID: 2272
• NCBI Accession #: NP_001159715.1
• NCBI GenBank Nucleotide #: NM_001166243.2
• UniProt Accession #: P49789; Q45QG9; Q6IU12; A2IAS9; A2IAT0; A2IAT6; A8K1A9
• Molecular Weight: 16,858 Da
• NCBI Official Full Name: bis(5'-adenosyl)-triphosphatase 1
• NCBI Official Synonym Full Names: fragile histidine triad
• NCBI Official Symbol: FHIT
• NCBI Official Synonym Symbols: FRA3B; AP3Aase
• NCBI Protein Information: bis(5'-adenosyl)-triphosphatase
• UniProt Protein Name: Bis(5'-adenosyl)-triphosphatase
• Protein Family: Bis(5'-adenosyl)-triphosphatase
• UniProt Gene Name: FHIT
• UniProt Synonym Gene Names: AP3Aase
• UniProt Entry Name: FHIT_HUMAN

Uniprot Description

FHIT: a member of the histidine triad gene family. A diadenosine 5',5'''-P1,P3-triphosphate hydrolase involved in purine metabolism. Its gene encompasses the common fragile site FRA3B on chromosome 3, where carcinogen-induced damage can lead to translocations and aberrant transcripts of this gene. A possible tumor suppressor in specific tissues. Phospho-FHIT observed in liver and kidney, but not in brain and lung. Phospho-FHIT undetected in all tested human tumor cell lines. Aberrant transcripts from this gene have been found in about half of all esophageal, stomach, and colon carcinomas.

Protein type: DNA replication; EC 3.6.1.29; Hydrolase; Motility/polarity/chemotaxis; Nucleotide Metabolism - purine; Tumor suppressor

Chromosomal Location of Human Ortholog: 3p14.2

Cellular Component: cytoplasm; cytosol

Molecular Function: bis(5'-adenosyl)-triphosphatase activity; catalytic activity; hydrolase activity; identical protein binding; protein binding; ubiquitin protein ligase binding

Biological Process: negative regulation of proteasomal ubiquitin-dependent protein catabolic process; nucleotide metabolic process; purine nucleotide metabolic process