RAP1A recombinant protein

Recombinant Human RAP1A, Member RAS Oncogene Family

Cat. Num. AAA144040

• Gene Names: RAP1A; RAP1; C21KG; G-22K; KREV1; KREV-1; SMGP21
• Purity: Greater than 85.0% as determined by SDS-PAGE.
• Synonyms: RAP1A; Recombinant Human RAP1A; Member RAS Oncogene Family; RAP1A Human; Member RAS Oncogene Family Human Recombinant; KREV1; G-22K; C21KG; RAP1; Ras-related protein Rap-1A; GTP-binding protein smg p21A; Ras-related protein Krev-1; RAP1A recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 85.0% as determined by SDS-PAGE.
• Form/Format: 0.25mg/ml solution containing 20mM Tris HCL pH-8.0, 5mM DTT, 0.2M NaCl & 40% glycerol.; Sterile filtered colorless solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MREYKLVVLG SGGVGKSALT VQFVQGIFVE KYDPTIEDSY RKQVEVDCQQ CMLEILDTAG TEQFTAMRDL YMKNGQGFAL VYSITAQSTF NDLQDLREQI LRVKDTEDVP MILVGNKCDL EDERVVGKEQ GQNLARQWCN CAFLESSAKS KINVNEIFYD LVRQINRKTP VEKKKPKKKS C
• Sequence Length: 184

• Preparation and Storage: RAP1A Human although stable at 4 degree C for 1 week, should be stored desiccated below -18 degree C. Please prevent freeze thaw cycles.

Related Product Information for RAP1A recombinant protein

Description: RAP1A Recombinant E Coli produced in E Coli is a single, non-glycosylated polypeptide chain containing 201 amino acids (1-181 a.a.) and having a molecular mass of 22.8 kDa. The RAP1A is fused to 20 amino acid His-Tag at N-terminus and purified by conventional chromatography.

Introduction: RAP1A is part of the family of RAS-related proteins which possess 50% amino acid identity with the classical RAS proteins and have various structural characteristics in common. RAP proteins differ from RAS proteins in their 61st amino acid, glutamine in RAS is replaced by threonine in RAP proteins. RAP1A induces morphological reversion of a cell line transformed by a Ras oncogene and counteracts the mitogenic function of Ras, since it can interact with Ras GAPs and RAF in a competitive manner.

Product Categories/Family for RAP1A recombinant protein

Recombinant & Natural Proteins; Recombinant Proteins; Member RAS Oncogene Family

NCBI and Uniprot Product Information

• NCBI GI #: 58331202
• NCBI GeneID: 5906
• NCBI Accession #: NP_001010935.1
• NCBI GenBank Nucleotide #: NM_001010935.2
• UniProt Accession #: P62834; P10113
• Molecular Weight: 20,987 Da
• NCBI Official Full Name: ras-related protein Rap-1A
• NCBI Official Synonym Full Names: RAP1A, member of RAS oncogene family
• NCBI Official Symbol: RAP1A
• NCBI Official Synonym Symbols: RAP1; C21KG; G-22K; KREV1; KREV-1; SMGP21
• NCBI Protein Information: ras-related protein Rap-1A; GTP-binding protein smg p21A; Ras-related protein Krev-1
• UniProt Protein Name: Ras-related protein Rap-1A
• Protein Family: Ras-related protein
• UniProt Gene Name: RAP1A
• UniProt Synonym Gene Names: KREV1
• UniProt Entry Name: RAP1A_HUMAN

NCBI Description

This gene encodes a member of the Ras family of small GTPases. The encoded protein undergoes a change in conformational state and activity, depending on whether it is bound to GTP or GDP. This protein is activated by several types of guanine nucleotide exchange factors (GEFs), and inactivated by two groups of GTPase-activating proteins (GAPs). The activation status of the encoded protein is therefore affected by the balance of intracellular levels of GEFs and GAPs. The encoded protein regulates signaling pathways that affect cell proliferation and adhesion, and may play a role in tumor malignancy. Pseudogenes of this gene have been defined on chromosomes 14 and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

Uniprot Description

RAP1A: a small G protein of the Ras family with tumor suppressor activity. Induces morphological reversion of a cell line transformed by a Ras oncogene. Activated by guanine nucleotide-exchange factors (GEF) EPAC and EPAC2 in a cAMP-dependent manner, and GFR. Counteracts the mitogenic function of Ras, at least partly because it can interact with Ras GAPs and RAF in a competitive manner. Interacts with SGSM1, SGSM2 and SGSM3.

Protein type: G protein, monomeric, Ras; G protein, monomeric; G protein; Tumor suppressor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1p13.3

Cellular Component: neuron projection; perinuclear region of cytoplasm; late endosome; early endosome; cytoplasm; plasma membrane; cytosol; cell junction

Molecular Function: GTPase activity; Rap guanyl-nucleotide exchange factor activity; protein binding; Ras GTPase binding; GTP binding; protein complex binding; protein transporter activity

Biological Process: platelet activation; microvillus biogenesis; protein transport; activation of MAPKK activity; nerve growth factor receptor signaling pathway; positive regulation of protein kinase activity; energy reserve metabolic process; signal transduction; blood coagulation; regulation of insulin secretion; Rap protein signal transduction