Western Blot (WB)
(RAP1A Antibody (C-term) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the RAP1A antibody detected the RAP1A protein (arrow).)
Rabbit RAP1A Polyclonal Antibody | anti-RAP1A antibody
RAP1A Antibody (C-term)
Western Blot (WB)
(RAP1A Antibody (C-term) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the RAP1A antibody detected the RAP1A protein (arrow).)
Western Blot (WB)
(RAP1A Antibody (C-term) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the RAP1A antibody detected the RAP1A protein (arrow).)
RAS-related proteins. These proteins share approximately 50% amino
acid identity with the classical RAS proteins and have numerous
structural features in common. The most striking difference between
RAP proteins and RAS proteins resides in their 61st amino acid:
glutamine in RAS is replaced by threonine in RAP proteins. The
product of this gene counteracts the mitogenic function of RAS
because it can interact with RAS GAPs and RAF in a competitive
manner. Two transcript variants encoding the same protein have been
identified for this gene.
Kelly, P., et al. J. Biol. Chem. 285(21):15777-15785(2010)
Ahmed, S.M., et al. J. Biol. Chem. 285(9):6538-6551(2010)
Hsu, Y.H., et al. PLoS Genet. 6 (6), E1000977 (2010) :
Sarthy, J., et al. EMBO J. 28(21):3390-3399(2009)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the Ras family of small GTPases. The encoded protein undergoes a change in conformational state and activity, depending on whether it is bound to GTP or GDP. This protein is activated by several types of guanine nucleotide exchange factors (GEFs), and inactivated by two groups of GTPase-activating proteins (GAPs). The activation status of the encoded protein is therefore affected by the balance of intracellular levels of GEFs and GAPs. The encoded protein regulates signaling pathways that affect cell proliferation and adhesion, and may play a role in tumor malignancy. Pseudogenes of this gene have been defined on chromosomes 14 and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]
Uniprot Description
RAP1A: a small G protein of the Ras family with tumor suppressor activity. Induces morphological reversion of a cell line transformed by a Ras oncogene. Activated by guanine nucleotide-exchange factors (GEF) EPAC and EPAC2 in a cAMP-dependent manner, and GFR. Counteracts the mitogenic function of Ras, at least partly because it can interact with Ras GAPs and RAF in a competitive manner. Interacts with SGSM1, SGSM2 and SGSM3.
Protein type: G protein, monomeric, Ras; Tumor suppressor; G protein; Motility/polarity/chemotaxis; G protein, monomeric
Chromosomal Location of Human Ortholog: 1p13.3
Cellular Component: neuron projection; perinuclear region of cytoplasm; late endosome; cytoplasm; early endosome; plasma membrane; cell junction; cytosol
Molecular Function: GTPase activity; Rap guanyl-nucleotide exchange factor activity; protein binding; Ras GTPase binding; GTP binding; protein complex binding; protein transporter activity
Biological Process: platelet activation; microvillus biogenesis; protein transport; nerve growth factor receptor signaling pathway; activation of MAPKK activity; positive regulation of protein kinase activity; energy reserve metabolic process; blood coagulation; signal transduction; regulation of insulin secretion; Rap protein signal transduction
Research Articles on RAP1A
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Product Notes
The RAP1A rap1a (Catalog #AAA9212242) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 142-170. The RAP1A Antibody (C-term) reacts with Mouse (Predicted Reactivity: Bovine, Rat, Chicken, Zebrafish, Human, Monkey, Xenopus) and may cross-react with other species as described in the data sheet. AAA Biotech's RAP1A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the RAP1A rap1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAP1A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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