Rabbit anti-Human, Mouse TUT1 Polyclonal Antibody | anti-TUT1 antibody

TUT1 Antibody

Cat. Num. AAA9409644

• Gene Names: TUT1; PAPD2; RBM21; URLC6; TUTase; STARPAP
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: TUT1; Polyclonal Antibody; TUT1 Antibody; U6 snRNA-specific terminal uridylyltransferase 1; EC 2.7.7.52; U6-TUTase; RNA-binding protein 21; RNA-binding motif protein 21; anti-TUT1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total TUT1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 912

• Applicable Applications for anti-TUT1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:500~1:3000; Immunohistochemistry: 1:50~1:100
• Immunogen Type: Peptide
• Immunogen Description: Synthesized peptide derived from internal of human TUT1.
• Target Name: TUT1
• Preparation and Storage: Store at -20°C

Western Blot (WB)

(Western blot analysis of extracts from 3T3 cells, using TUT1 antiobdy (MBS9409644).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using TUT1 antibody (MBS9409644).)

Related Product Information for anti-TUT1 antibody

Poly(A) polymerase that creates the 3'-poly(A) tail of specific pre-mRNAs. Localizes to nuclear speckles together with PIP5K1A and mediates polyadenylation of a select set of mRNAs, such as HMOX1. In addition to polyadenylation, it is also required for the 3'-end cleavage of pre-mRNAs: binds to the 3'UTR of targeted pre-mRNAs and promotes the recruitment and assembly of the CPSF complex on the 3'UTR of pre-mRNAs. In addition to adenylyltransferase activity, also has uridylyltransferase activity. However, the ATP ratio is higher than UTP in cells, suggesting that it functions primarily as a poly(A) polymerase. Acts as a specific terminal uridylyltransferase for U6 snRNA in vitro: responsible for a controlled elongation reaction that results in the restoration of the four 3'-terminal UMP-residues found in newly transcribed U6 snRNA. Not involved in replication-dependent histone mRNA degradation.Ota T., Nat. Genet. 36:40-45(2004).The MGC Project Team, Genome Res. 14:2121-2127(2004).Trippe R.,RNA 12:1494-1504(2006).

Product Categories/Family for anti-TUT1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 226371750
• NCBI GeneID: 64852
• NCBI Accession #: NP_073741.2
• NCBI GenBank Nucleotide #: NM_022830.2
• UniProt Accession #: Q9H6E5; Q2NL65; Q7L583; Q9H6H7; A1A527; A8K995
• Molecular Weight: 95kd
• NCBI Official Full Name: speckle targeted PIP5K1A-regulated poly(A) polymerase
• NCBI Official Synonym Full Names: terminal uridylyl transferase 1, U6 snRNA-specific
• NCBI Official Symbol: TUT1
• NCBI Official Synonym Symbols: PAPD2; RBM21; URLC6; TUTase; STARPAP
• NCBI Protein Information: speckle targeted PIP5K1A-regulated poly(A) polymerase
• UniProt Protein Name: Speckle targeted PIP5K1A-regulated poly(A) polymerase
• Protein Family: Speckle targeted PIP5K1A-regulated poly(A) polymerase
• UniProt Gene Name: TUT1
• UniProt Synonym Gene Names: RBM21; Star-PAP; RNA-binding protein 21; U6-TUTase
• UniProt Entry Name: STPAP_HUMAN

NCBI Description

This gene encodes a nucleotidyl transferase that functions as both a terminal uridylyltransferase and a nuclear poly(A) polymerase. The encoded enzyme specifically adds and removes nucleotides from the 3' end of small nuclear RNAs and select mRNAs and may function in controlling gene expression and cell proliferation.[provided by RefSeq, Apr 2009]

Uniprot Description

RBM21: Highly specific terminal uridylyltransferase that exclusively accepts U6 snRNA as substrate. U6 snRNA is unique in that nucleotides are both added to and removed from its 3'-end. U6-TUTase is responsible for a controlled elongation reaction that results in the restoration of the four 3'-terminal UMP-residues found in newly transcribed U6 snRNA. Does not play a role in replication-dependent histone mRNA degradation.

Protein type: Nucleolus; EC 2.7.7.52; EC 2.7.7.19; Transferase

Chromosomal Location of Human Ortholog: 11q12.2

Cellular Component: nucleoplasm; intercellular bridge; nucleolus; nuclear speck; mRNA cleavage and polyadenylation specificity factor complex

Molecular Function: polynucleotide adenylyltransferase activity; protein binding; enzyme binding; mRNA 3'-UTR binding; RNA binding; metal ion binding; RNA uridylyltransferase activity; ATP binding

Biological Process: snRNA processing; mRNA polyadenylation; mRNA cleavage