Non-Metastatic Cells 4 Active Protein | NME4 active protein

Recombinant Human Non-Metastatic Cells 4, BioActive

Cat. Num. AAA141638

• Gene Names: NME4; NDPK-D; NM23H4; nm23-H4
• Purity: Greater than 90.0% as determined by SDS-PAGE.
• Synonyms: Non-Metastatic Cells 4; Recombinant Human Non-Metastatic Cells 4; BioActive; NME4 Active; NME4 Human Active; Non-Metastatic Cells 4 Human Recombinant; Nucleoside diphosphate kinase mitochondrial; Nucleoside diphosphate kinase; mitochondrial; NDK; NDPKD; nm23-H4; NM23D; NME4 active protein

• Host: E Coli
• Purity/Purification: Greater than 90.0% as determined by SDS-PAGE.
• Form/Format: Sterile filtered colorless solution.; The NME4 solution (0.5mg/ml) contains 40% glycerol, 20mM Tris-HCl buffer (pH8.0) and 0.2M NaCl.
• Sequence: MGSSHHHHHH SSGLVPRGSH MPSWTRERTL VAVKPDGVQR RLVGDVIQRF ERRGFTLVGM KMLQAPESVL AEHYQDLRRK PFYPALIRYM SSGPVVAMVW EGYNVVRASR AMIGHTDSAE AAPGTIRGDF SVHISRNVIH ASDSVEGAQR EIQLWFQSSE LVSWADGGQH SSIHPA

• Biological Activity: Specific activity is >120unit/mg, and is defined as the amount of enzyme that convert 1.0 umole each of ATP and TDP to ADP and TTP per minute at pH7.5 at 25 degree C in a couple system with PK/LDH.
• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks.; Store, frozen at -20 degree C for longer periods of time.; For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).; Avoid multiple freeze-thaw cycles.

Related Product Information for NME4 active protein

Non-Metastatic Cells 4 or NME4, is a nucleoside diphosphate kinase located in the mitochondria, and is part of the NDK family of proteins. NME4 ais a very common enzyme that enhances transfer of gamma-phosphates, through a phosphohistidine as a intermediate, between dioxynucleoside tri- and diphosphates. NME4 is originated from the nm23 gene. NME4 has a crucial part in the creation of nucleoside triphosphates that are not ATP.
NME4 Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 176 amino acids (33-187a.a.) and having a molecular mass of 19.6kDa.NME4 is fused to a 21 amino acid His tag at N-Terminus and purified by proprietary chromatographic techniques.

Product Categories/Family for NME4 active protein

RECOMBINANT & NATURAL PROTEINS

NCBI and Uniprot Product Information

• NCBI GI #: 556695376
• NCBI GeneID: 4833
• NCBI Accession #: NP_001273362.1
• NCBI GenBank Nucleotide #: NM_001286433.1
• UniProt Accession #: O00746; Q5U0M9; A2IDD0
• Molecular Weight: 12,979 Da
• NCBI Official Full Name: nucleoside diphosphate kinase, mitochondrial isoform b
• NCBI Official Synonym Full Names: NME/NM23 nucleoside diphosphate kinase 4
• NCBI Official Symbol: NME4
• NCBI Official Synonym Symbols: NDPK-D; NM23H4; nm23-H4
• NCBI Protein Information: nucleoside diphosphate kinase, mitochondrial; NDK; NDP kinase D; NDP kinase, mitochondrial; NDPKD; non-metastatic cells 4, protein expressed in; nucleoside diphosphate kinase D
• UniProt Protein Name: Nucleoside diphosphate kinase, mitochondrial
• UniProt Gene Name: NME4
• UniProt Synonym Gene Names: NM23D; NDK; NDP kinase, mitochondrial; NDPKD
• UniProt Entry Name: NDKM_HUMAN

NCBI Description

The nucleoside diphosphate (NDP) kinases (EC 2.7.4.6) are ubiquitous enzymes that catalyze transfer of gamma-phosphates, via a phosphohistidine intermediate, between nucleoside and dioxynucleoside tri- and diphosphates. The enzymes are products of the nm23 gene family, which includes NME4 (Milon et al., 1997 [PubMed 9099850]).[supplied by OMIM, May 2008]

Uniprot Description

NME4: Major role in the synthesis of nucleoside triphosphates other than ATP. Belongs to the NDK family.

Protein type: Kinase, other; Nucleotide Metabolism - purine; EC 2.7.4.6; Kinase, nucleoside diphosphate; Nucleotide Metabolism - pyrimidine; Mitochondrial; Other group; NDK family

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: mitochondrion; mitochondrial inner membrane; mitochondrial intermembrane space; cytosol

Molecular Function: protein binding; metal ion binding; nucleoside diphosphate kinase activity; protein complex binding; ATP binding

Biological Process: regulation of apoptosis; GTP biosynthetic process; CTP biosynthetic process; nucleobase, nucleoside and nucleotide metabolic process; pyrimidine nucleotide metabolic process; nucleobase, nucleoside and nucleotide interconversion; UTP biosynthetic process; nucleoside triphosphate biosynthetic process; nucleoside metabolic process; nucleoside diphosphate phosphorylation; purine nucleotide metabolic process