NME4 active protein

NME4, human recombinant

Cat. Num. AAA845318

• Gene Names: NME4; NDPK-D; NM23H4; nm23-H4
• Applications: SDS-Page
• Purity: >=90%
• Synonyms: NME4; human recombinant; Nucleoside diphosphate kinase; mitochondrial; NDK; NDPKD; nm23-H4; NM23D; NME4 active protein

• Host: E Coli
• Purity/Purification: >=90%
• Form/Format: 0.5 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, and 0.2 M NaCl ; Appearance: Liquid
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MPSWTRERTL VAVKPDGVQR RLVGDVIQRF ERRGFTLVGM KMLQAPESVL AEHYQDLRRK PFYPALIRYM SSGPVVAMVW EGYNVVRASR AMIGHTDSAE AAPGTIRGDF SVHISRNVIH ASDSVEGAQR EIQLWFQSSE LVSWADGGQH SSIHPA
• Sequence Length: 153

• Applicable Applications for NME4 active protein: SDS-PAGE
• Biological Activity: Specific activity is > 15 units/ml, in which one unit will convert 1.0 umole each of TDP and ATP to TTP and ADP per minute at pH 7.6 at 25 degree C in a coupled system with PK/LDH.
• Results: Specific activity is > 15 units/ml
• Unit Definition: One unit will convert 1.0 umole each of TDP and ATP to TTP and ADP per minute at pH 7.6 at 25 degree C in a coupled system with PK/LDH.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for NME4 active protein

Background: NME4, also known as nucleoside diphosphate kinase, mitochondrial, belongs to the NDK family. NME4 are ubiquitous enzymes that catalyze transfer of gamma-phosphates, via a phosphohistidine intermediate, between nucleoside and dioxynucleoside tri- and diphosphates. The enzymes are products of the nm23 gene family, which includes NME4. NME4 plays a major role in the synthesis of nucleoside triphosphates other than ATP. Recombinant human NME4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Product Categories/Family for NME4 active protein

Proteins and Enzymes; Proteins and Enzymes (A-Z)

NCBI and Uniprot Product Information

• NCBI GI #: 556695376
• NCBI GeneID: 4833
• NCBI Accession #: NP_001273362.1
• NCBI GenBank Nucleotide #: NM_001286433.1
• UniProt Accession #: O00746; Q5U0M9; A2IDD0
• Molecular Weight: 19.6 kDa (176 aa, 33-187 aa + His Tag)
• NCBI Official Full Name: nucleoside diphosphate kinase, mitochondrial isoform b
• NCBI Official Synonym Full Names: NME/NM23 nucleoside diphosphate kinase 4
• NCBI Official Symbol: NME4
• NCBI Official Synonym Symbols: NDPK-D; NM23H4; nm23-H4
• NCBI Protein Information: nucleoside diphosphate kinase, mitochondrial
• UniProt Protein Name: Nucleoside diphosphate kinase, mitochondrial
• UniProt Gene Name: NME4
• UniProt Synonym Gene Names: NM23D; NDK; NDP kinase, mitochondrial; NDPKD
• UniProt Entry Name: NDKM_HUMAN

NCBI Description

The nucleoside diphosphate (NDP) kinases (EC 2.7.4.6) are ubiquitous enzymes that catalyze transfer of gamma-phosphates, via a phosphohistidine intermediate, between nucleoside and dioxynucleoside tri- and diphosphates. The enzymes are products of the nm23 gene family, which includes NME4 (Milon et al., 1997 [PubMed 9099850]).[supplied by OMIM, May 2008]

Uniprot Description

NME4: Major role in the synthesis of nucleoside triphosphates other than ATP. Belongs to the NDK family.

Protein type: EC 2.7.4.6; Nucleotide Metabolism - purine; Kinase, other; Kinase, nucleoside diphosphate; Mitochondrial; Nucleotide Metabolism - pyrimidine; Other group; NDK family

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: mitochondrion; mitochondrial inner membrane; mitochondrial intermembrane space; cytosol

Molecular Function: protein binding; metal ion binding; protein complex binding; nucleoside diphosphate kinase activity; ATP binding

Biological Process: regulation of apoptosis; GTP biosynthetic process; CTP biosynthetic process; pyrimidine nucleotide metabolic process; nucleobase, nucleoside and nucleotide metabolic process; nucleobase, nucleoside and nucleotide interconversion; UTP biosynthetic process; nucleoside triphosphate biosynthetic process; nucleoside metabolic process; nucleoside diphosphate phosphorylation; purine nucleotide metabolic process