Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human breast cancer using MBS7044024 at dilution 1:100)
Rabbit anti-Human NME4 Polyclonal Antibody | anti-NME4 antibody
NME4 Antibody
Gene Names
NME4; NDPK-D; NM23H4; nm23-H4
Reactivity
Human
Applications
ELISA, Immunohistochemistry
Purity
Antigen Affinity Purified
Synonyms
NME4; Polyclonal Antibody; NME4 Antibody; Nucleoside diphosphate kinase; mitochondrial; NDK; NDP kinase; Nucleoside diphosphate kinase D; NDPKD; nm23-H4; NM23D; anti-NME4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid
Sequence Length
153
Applicable Applications for anti-NME4 antibody
ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
Recommended dilution: IHC: 1:20-1:200
Species
Human
Immunogen
Recombinant human Nucleoside diphosphate kinase, mitochondrial protein (1-187AA)
Conjugate
Non-conjugated
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-50948 / sc-50951 / sc-99071
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human breast cancer using MBS7044024 at dilution 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human breast cancer using MBS7044024 at dilution 1:100)
Related Product Information for anti-NME4 antibody
Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Through the catalyzed exchange of gamma-phosphate between di- and triphosphonucleosides participates in regulation of intracellular nucleotide homeostasis (PubMed:10799505). Binds to anionic phospholipids, predominantly to cardiolipin; the binding inhibits its phosphotransfer activity (PubMed:18635542, PubMed:23150663). Acts as mitochondria-specific NDK; its association with cardiolipin-containing mitochondrial inner membrane is coupled to respiration suggesting that ADP locally regenerated in the mitochondrion innermembrane space by its activity is directly taken up via ANT ADP/ATP translocase into the matrix space to stimulate respiratory ATP regeneration (PubMed:18635542). Proposed to increase GTP-loading on dynamin-related GTPase OPA1 in mitochondria (PubMed:24970086). In vitro can induce liposome cross-linking suggesting that it can cross-link inner and outer membranes to form contact sites, and promotes intermembrane migration of anionic phosphoplipids. Promotes the redistribution of cardiolipin between the mitochondrial inner membrane and outer membrane which is implicated in pro-apoptotic signaling (PubMed:18635542, PubMed:17028143, PubMed:23150663).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
12,979 Da
NCBI Official Full Name
nucleoside diphosphate kinase, mitochondrial isoform b
NCBI Official Synonym Full Names
NME/NM23 nucleoside diphosphate kinase 4
NCBI Official Symbol
NME4
NCBI Official Synonym Symbols
NDPK-D; NM23H4; nm23-H4
NCBI Protein Information
nucleoside diphosphate kinase, mitochondrial
UniProt Protein Name
Nucleoside diphosphate kinase, mitochondrial
UniProt Gene Name
NME4
UniProt Synonym Gene Names
NM23D; NDK; NDP kinase, mitochondrial; NDPKD
UniProt Entry Name
NDKM_HUMAN
NCBI Description
The nucleoside diphosphate (NDP) kinases (EC 2.7.4.6) are ubiquitous enzymes that catalyze transfer of gamma-phosphates, via a phosphohistidine intermediate, between nucleoside and dioxynucleoside tri- and diphosphates. The enzymes are products of the nm23 gene family, which includes NME4 (Milon et al., 1997 [PubMed 9099850]).[supplied by OMIM, May 2008]
Uniprot Description
NME4: Major role in the synthesis of nucleoside triphosphates other than ATP. Belongs to the NDK family.
Protein type: Nucleotide Metabolism - pyrimidine; Nucleotide Metabolism - purine; EC 2.7.4.6; Kinase, other; Kinase, nucleoside diphosphate; Mitochondrial; Other group; NDK family
Chromosomal Location of Human Ortholog: 16p13.3
Cellular Component: mitochondrial inner membrane; mitochondrion
Molecular Function: nucleoside diphosphate kinase activity; protein binding
Biological Process: nucleobase, nucleoside and nucleotide interconversion; nucleoside metabolic process; nucleoside triphosphate biosynthetic process; purine nucleotide metabolic process; pyrimidine nucleotide metabolic process; regulation of apoptosis
Research Articles on NME4
Similar Products
Product Notes
The NME4 nme4 (Catalog #AAA7044024) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NME4 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NME4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC). Recommended dilution: IHC: 1:20-1:200. Researchers should empirically determine the suitability of the NME4 nme4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NME4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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