Human Epigen (EPG) CLIA Kit | EPG clia kit

Human Epigen (EPG) CLIA Kit

Cat. Num. AAA2709236

• Reactivity: Human
• Applications: Chemiluminescence Immunoassay (CLIA)
• Synonyms: Epigen (EPG); Human Epigen (EPG) CLIA Kit; Epigen; EPGN; Epithelial Mitogen Homolog; EPG clia kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Epigen (EPG).; No significant cross-reactivity or interference between Epigen (EPG) and analogues was observed.
• Sequence Length: 154

• Applicable Applications for EPG clia kit: Chemiluminescence Immunoassay (CLIA)
• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, cell lysates and other biological fluids
• Detection Range: 27.4-20,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 11.2pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Epigen (EPG) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Epigen (EPG) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Epigen (EPG). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Epigen (EPG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Epigen (EPG) level in the sample or standard.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 401782583
• NCBI Accession #: NP_001257918.1
• NCBI GenBank Nucleotide #: NP_001257918.1
• UniProt Accession #: Q6UW88
• NCBI Official Full Name: epigen isoform 1
• UniProt Protein Name: Epigen
• UniProt Gene Name: EPGN
• UniProt Synonym Gene Names: EPG
• UniProt Entry Name: EPGN_HUMAN

Uniprot Description

Epigen: Promotes the growth of epithelial cells. May stimulate the phosphorylation of EGFR and mitogen-activated protein kinases. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Ligand, receptor tyrosine kinase; Membrane protein, integral

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: extracellular space; integral to plasma membrane

Molecular Function: growth factor activity; epidermal growth factor receptor binding

Biological Process: positive regulation of MAP kinase activity; positive regulation of mitosis; positive regulation of epidermal growth factor receptor activity; activation of MAPK activity; positive regulation of cell proliferation; MAPKKK cascade; angiogenesis; positive regulation of epithelial cell proliferation