Testing Data
anti-Rat 5-HT (Serotonin) Transporter Antibody | anti-5HT antibody
5-HT (Serotonin) Transporter Antibody, Rabbit
Reactivity
Rat
Applications
Immunohistochemistry, Western Blot
Synonyms
5-HT (Serotonin) Transporter; 5-HT (Serotonin) Transporter Antibody; Rabbit; anti-5HT antibody
Reactivity
Rat
Form/Format
Whole serum - Lyophilized
Applicable Applications for anti-5HT antibody
Immunohistochemistry (IHC), Western Blot (WB)
Type Info
Rabbit IgG
Immunogen
synthetic peptide sequence corresponding to amino acids 579-599 of rat 5HT transporter coupled to KLH.
Preparation and Storage
Store at 4-8 degrees
Testing Data
Testing Data
Related Product Information for anti-5HT antibody
The serotonin transporter (SERT) is a monoamine transporter, integral membrane protein that transports the neurotransmitter serotonin from synaptic spaces into presynaptic neurons. This transport of serotonin by the SERT protein terminates the action of serotonin and recycles it in a sodium-dependent manner.
The histochemical antibody for serotonin (5HT) transporter is generated in a rabbit against a synthetic peptide sequence corresponding to amino acids 579-599 of rat 5HT transporter coupled to KLH.
The antibody produces 4+ (maximum) labeling of 5HT transporter at dilutions of 1/200 - 1/400 using indirect immunofluorescence and at dilutions of 1/10,000 - 1/15,000 using biotin/streptavidin HRP technique in rat raphe nuclei, hypothalamus, cortex, and spinal cord. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended. By Western blot analysis using rat brain extracts of cortex, hypothalamus, midbrain, and hindbrain, the antibody specifically labels a single band. Immunolabeling is completely abolished by pre-adsorption with synthetic rat 5HT transporter.
The histochemical antibody for serotonin (5HT) transporter is generated in a rabbit against a synthetic peptide sequence corresponding to amino acids 579-599 of rat 5HT transporter coupled to KLH.
The antibody produces 4+ (maximum) labeling of 5HT transporter at dilutions of 1/200 - 1/400 using indirect immunofluorescence and at dilutions of 1/10,000 - 1/15,000 using biotin/streptavidin HRP technique in rat raphe nuclei, hypothalamus, cortex, and spinal cord. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended. By Western blot analysis using rat brain extracts of cortex, hypothalamus, midbrain, and hindbrain, the antibody specifically labels a single band. Immunolabeling is completely abolished by pre-adsorption with synthetic rat 5HT transporter.
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Product Notes
The 5HT (Catalog #AAA555180) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The 5-HT (Serotonin) Transporter Antibody, Rabbit reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's 5-HT (Serotonin) Transporter can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Western Blot (WB). Researchers should empirically determine the suitability of the 5HT for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "5-HT (Serotonin) Transporter, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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