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Testing Data

Rabbit SR-5A Polyclonal Antibody | anti-SR-5A antibody

SR-5A (L35) Polyclonal Antibody

Gene Names
HTR5A; 5-HT5A
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunofluorescence
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
SR-5A; Polyclonal Antibody; SR-5A (L35) Polyclonal Antibody; HTR5A; Serotonin receptor 5A; 5-HT5A; 5-HT-5A; 5-hydroxytryptamine receptor 5A; anti-SR-5A antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
SR-5A (L35) polyclonal antibody detects endogenous levels of SR-5A protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
357
Applicable Applications for anti-SR-5A antibody
Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
IHC: 1:50-1:200
IF: 1:50-1:200
Immunogen
Synthetic peptide, corresponding to amino acids N-terminus of Human SR-5A.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.

Testing Data

Testing Data
Related Product Information for anti-SR-5A antibody
Serotonin actions are mediated by receptors that influence the biochemistry of depression, anxiety, sexuality and appetite. Rat SR-5B is present in serotonergic neurons in dorsal raphe (DR) and central superior nucleus (CS, median raphe nucleus). DR cell bodies showing SR-5B mRNA expression are abundant in the medial portions of the nucleus. CS coexpression of SR-5B receptor mRNA with Serotonin transporter mRNA is high in the intermediate portions of the nucleus. Serotonin receptors include SR-1-7 (5HT1-7). Subtypes within the SR-1 group include SR-1A, -1B, -1D, -1E and -1F. Subtypes within the SR-2 group include SR-2A, -2B and -2C. Subtypes within the SR-5 group include SR-5A and -5B.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 40kDa
NCBI Official Full Name
5-hydroxytryptamine receptor 5A
NCBI Official Synonym Full Names
5-hydroxytryptamine receptor 5A
NCBI Official Symbol
HTR5A
NCBI Official Synonym Symbols
5-HT5A
NCBI Protein Information
5-hydroxytryptamine receptor 5A
UniProt Protein Name
5-hydroxytryptamine receptor 5A
UniProt Gene Name
HTR5A
UniProt Synonym Gene Names
5-HT-5; 5-HT-5A; 5-HT5A
UniProt Entry Name
5HT5A_HUMAN

NCBI Description

The neurotransmitter serotonin (5-hydroxytryptamine, 5-HT) has been implicated in a wide range of psychiatric conditions and also has vasoconstrictive and vasodilatory effects. The gene described in this record is a member of 5-hydroxytryptamine (serotonin) receptor family and encodes a multi-pass membrane protein that functions as a receptor for 5-hydroxytryptamine and couples to G-proteins. This protein has been shown to function in part through the regulation of intracellular Ca2+ mobilization. [provided by RefSeq, Jul 2008]

Uniprot Description

5-HT(5A): This is one of the several different receptors for 5- hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins. Belongs to the G-protein coupled receptor 1 family.

Protein type: GPCR, family 1; Receptor, GPCR; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 7q36.1

Cellular Component: Golgi apparatus; rough endoplasmic reticulum; integral to plasma membrane; dendrite; plasma membrane; perikaryon

Molecular Function: serotonin receptor activity

Biological Process: G-protein signaling, coupled to cyclic nucleotide second messenger; G-protein coupled receptor protein signaling pathway; cAMP-mediated signaling; hippocampus development; serotonin receptor signaling pathway; response to estradiol stimulus

Research Articles on SR-5A

Similar Products

Product Notes

The SR-5A htr5a (Catalog #AAA3003024) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SR-5A (L35) Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SR-5A can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Immunofluorescence (IF). IHC: 1:50-1:200 IF: 1:50-1:200. Researchers should empirically determine the suitability of the SR-5A htr5a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SR-5A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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