Testing Data
(Image: 5HT Staining of rat hypothalamus.)
Goat 5-HT (Serotonin) receptor Antibody | anti-5HT antibody
5-HT (Serotonin) receptor, goat
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Synonyms
5-HT (Serotonin) receptor; goat; anti-5HT antibody
Host
Goat
Reactivity
Human, Mouse, Rat
Form/Format
100ul. Lyophilized
Applicable Applications for anti-5HT antibody
Immunohistochemistry; Western Blot
Application Notes
Immunohistochemistry:
Recommended Dilution: 1/400 - 1/800 in PBS/0.3% Triton X-100 – Cy3 Technique; 1/5000 - 1/10,000 in PBS/0.3% Triton X-100 – Bn/Av-HRP
Western Blot:
Recommended Dilution: 1/500 or greater Dilutions listed as a recommendation. Optimal dilution should be determined by investigator.
Immunohistochemistry:
Antigen: serotonin coupled to BSA with paraformaldehyde. Raised in goat
Control Tissue: Rat hypothalamus, raphe nuclei and spinal cord.
Perfusion Fixation: Fixative - 4% paraformaldehyde in 0.1 M Phosphate buffer, p 7.4; 500 mL over approximately 20 minutes.
Post Fixation: 4% paraformaldehyde in 0.1 M Phosphate buffer, pH 7.4; 500 mL over approximately 20 s. Post Fixation -1.5 hour at 4 degree C. in 4% paraformaldehyde in 0.1 M phosphate buffer, pH 7.4.
Note: Paraformaldehyde is a necessary component in fixation of serotonin. If needed, low levels of glutaraldehyde (0.1-0.3%) may be used in conjunction with paraformaldehyde.
Sections: 10um cryostat or 50um vibratome.
Antibody dilution: 1/400-1/800 in PBS/0.3% Triton X-100 - Cy3 Technique; 1/5,000 - 1/10,000 in PBS/0.3% Triton X-100 -Bn-SA/HRP Technique
Absorption control: 5-HT/BSA 25ug/mL diluted serum.
Incubation on Tissue: 16-24 hours at 4 degree C.
Detection System: use Cy3 and Bn-SA/HRP at dilutions recommended by the manufacturers.
Recommended Dilution: 1/400 - 1/800 in PBS/0.3% Triton X-100 – Cy3 Technique; 1/5000 - 1/10,000 in PBS/0.3% Triton X-100 – Bn/Av-HRP
Western Blot:
Recommended Dilution: 1/500 or greater Dilutions listed as a recommendation. Optimal dilution should be determined by investigator.
Immunohistochemistry:
Antigen: serotonin coupled to BSA with paraformaldehyde. Raised in goat
Control Tissue: Rat hypothalamus, raphe nuclei and spinal cord.
Perfusion Fixation: Fixative - 4% paraformaldehyde in 0.1 M Phosphate buffer, p 7.4; 500 mL over approximately 20 minutes.
Post Fixation: 4% paraformaldehyde in 0.1 M Phosphate buffer, pH 7.4; 500 mL over approximately 20 s. Post Fixation -1.5 hour at 4 degree C. in 4% paraformaldehyde in 0.1 M phosphate buffer, pH 7.4.
Note: Paraformaldehyde is a necessary component in fixation of serotonin. If needed, low levels of glutaraldehyde (0.1-0.3%) may be used in conjunction with paraformaldehyde.
Sections: 10um cryostat or 50um vibratome.
Antibody dilution: 1/400-1/800 in PBS/0.3% Triton X-100 - Cy3 Technique; 1/5,000 - 1/10,000 in PBS/0.3% Triton X-100 -Bn-SA/HRP Technique
Absorption control: 5-HT/BSA 25ug/mL diluted serum.
Incubation on Tissue: 16-24 hours at 4 degree C.
Detection System: use Cy3 and Bn-SA/HRP at dilutions recommended by the manufacturers.
Immunogen Sequence
Whole molecule 5HT
Preparation and Storage
Storage: Dilute with phosphate buffer or Tris buffer at dilutions no higher than 1/10, aliquot and freeze at -15° C or lower. Antibody can be stored for up to six months if handled as described above.
It is strongly recommended that the customer perform a primary antibody dilution series using our dilution recommendations as a guideline. Note that a change in the fixation or buffering system as used in our protocol may change the configuration.
It is strongly recommended that the customer perform a primary antibody dilution series using our dilution recommendations as a guideline. Note that a change in the fixation or buffering system as used in our protocol may change the configuration.
Testing Data
(Image: 5HT Staining of rat hypothalamus.)
Testing Data
(Image: 5HT Staining of rat hypothalamus.)
Related Product Information for anti-5HT antibody
The histochemical antibody for serotonin is generated in a goat against serotonin conjugated to bovine serum albumin with paraformaldehyde.
The antibody has a proven 4+ indirect immunofluorescence staining at a 1/400 - 1/800 dilution and a 4+ biotin-streptavidin/HRP staining at a 1/4000 - 1/10,000 dilution in rat hypothalamus and spinal cord.
The antibody has a proven 4+ indirect immunofluorescence staining at a 1/400 - 1/800 dilution and a 4+ biotin-streptavidin/HRP staining at a 1/4000 - 1/10,000 dilution in rat hypothalamus and spinal cord.
Product Categories/Family for anti-5HT antibody
Similar Products
Product Notes
The 5HT (Catalog #AAA555344) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The 5-HT (Serotonin) receptor, goat reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's 5-HT (Serotonin) receptor can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry; Western Blot. Immunohistochemistry: Recommended Dilution: 1/400 - 1/800 in PBS/0.3% Triton X-100 – Cy3 Technique; 1/5000 - 1/10,000 in PBS/0.3% Triton X-100 – Bn/Av-HRP Western Blot: Recommended Dilution: 1/500 or greater Dilutions listed as a recommendation. Optimal dilution should be determined by investigator. Immunohistochemistry: Antigen: serotonin coupled to BSA with paraformaldehyde. Raised in goat Control Tissue: Rat hypothalamus, raphe nuclei and spinal cord. Perfusion Fixation: Fixative - 4% paraformaldehyde in 0.1 M Phosphate buffer, p 7.4; 500 mL over approximately 20 minutes. Post Fixation: 4% paraformaldehyde in 0.1 M Phosphate buffer, pH 7.4; 500 mL over approximately 20 s. Post Fixation -1.5 hour at 4 degree C. in 4% paraformaldehyde in 0.1 M phosphate buffer, pH 7.4. Note: Paraformaldehyde is a necessary component in fixation of serotonin. If needed, low levels of glutaraldehyde (0.1-0.3%) may be used in conjunction with paraformaldehyde. Sections: 10um cryostat or 50um vibratome. Antibody dilution: 1/400-1/800 in PBS/0.3% Triton X-100 - Cy3 Technique; 1/5,000 - 1/10,000 in PBS/0.3% Triton X-100 -Bn-SA/HRP Technique Absorption control: 5-HT/BSA 25ug/mL diluted serum. Incubation on Tissue: 16-24 hours at 4 degree C. Detection System: use Cy3 and Bn-SA/HRP at dilutions recommended by the manufacturers. . Researchers should empirically determine the suitability of the 5HT for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "5-HT (Serotonin) receptor, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
