Human Proteolipid Protein 1, Myelin ELISA Kit | PLP1 elisa kit

Human Proteolipid Protein 1, Myelin ELISA Kit

Cat. Num. AAA733199

• Gene Names: PLP1; PLP; PMD; HLD1; MMPL; SPG2; GPM6C; PLP/DM20
• Reactivity: Human
• Synonyms: Proteolipid Protein 1; Myelin; Human Proteolipid Protein 1; Myelin ELISA Kit; PLP1 elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive or Sandwich
• Detection Range: 2.5-50ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This PLP1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PLP1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PLP1 elisa kit

Principle of the Assay: PLP1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PLP1. Standards or samples are then added to the microtiter plate wells and PLP1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PLP1 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for PLP1 are added to each well to "sandwich" the PLP1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PLP1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PLP1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for PLP1 elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 49168568
• NCBI GeneID: 5354
• UniProt Accession #: P60201; P04400; P06905; Q502Y1; Q6FHZ6
• Molecular Weight: 26,274 Da
• NCBI Official Full Name: PLP1
• NCBI Official Synonym Full Names: proteolipid protein 1
• NCBI Official Symbol: PLP1
• NCBI Official Synonym Symbols: PLP; PMD; HLD1; MMPL; SPG2; GPM6C; PLP/DM20
• NCBI Protein Information: myelin proteolipid protein; lipophilin; major myelin proteolipid protein
• UniProt Protein Name: Myelin proteolipid protein
• Protein Family: Patatin-like protein
• UniProt Gene Name: PLP1
• UniProt Synonym Gene Names: PLP; PLP
• UniProt Entry Name: MYPR_HUMAN

NCBI Description

This gene encodes a transmembrane proteolipid protein that is the predominant myelin protein present in the central nervous system. It may play a role in the compaction, stabilization, and maintenance of myelin sheaths, as well as in oligodendrocyte development and axonal survival. Mutations in this gene cause X-linked Pelizaeus-Merzbacher disease and spastic paraplegia type 2. Alternatively spliced transcript variants encoding distinct isoforms or having different 5' UTRs, have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

PLP1: This is the major myelin protein from the central nervous system. It plays an important role in the formation or maintenance of the multilamellar structure of myelin. Defects in PLP1 are the cause of leukodystrophy hypomyelinating type 1 (HLD1); also known as Pelizaeus-Merzbacher disease. HLD1 is an X-linked recessive dysmyelinating disorder of the central nervous system in which myelin is not formed properly. It is characterized clinically by nystagmus, spastic quadriplegia, ataxia, and developmental delay. Defects in PLP1 are the cause of spastic paraplegia X- linked type 2 (SPG2). SPG2 is characterized by spastic gait and hyperreflexia. In some patients, complicating features include nystagmus, dysarthria, sensory disturbance, mental retardation, optic atrophy. Belongs to the myelin proteolipid protein family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Cell surface

Chromosomal Location of Human Ortholog: Xq22

Cellular Component: integral to membrane; plasma membrane; myelin sheath

Molecular Function: structural constituent of myelin sheath; structural molecule activity

Biological Process: integrin-mediated signaling pathway; synaptic transmission; substantia nigra development; myelination in the central nervous system; cell maturation; long-chain fatty acid biosynthetic process; axon ensheathment; inflammatory response; astrocyte development

Disease: Spastic Paraplegia 2, X-linked; Pelizaeus-merzbacher Disease