Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Zinc transporter 8 (SLC30A8) Standard Curve.)
Human Zinc Transporter 8 (SLC30A8) ELISA Kit | ZNT8 elisa kit
Human Zinc Transporter 8 (SLC30A8) ELISA Kit
Gene Names
SLC30A8; ZNT8; ZnT-8
Reactivity
Human
Synonyms
Zinc Transporter 8 (SLC30A8); Human Zinc Transporter 8 (SLC30A8) ELISA Kit; SLC30A8; ZnT-8; solute carrier family 30 member 8; zinc transporter ZnT-8; ZNT8 elisa kit
Reactivity
Human
Specificity
Human Zinc transporter 8 (SLC30A8) ELISA Kit has high sensitivity and excellent specificity for detection of Human SLC30A8. No significant cross-reactivity or interference between Human SLC30A8 and analogues was observed.
Sequence Length
369
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Zinc transporter 8 (SLC30A8) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Zinc transporter 8 (SLC30A8) Standard Curve.)
Related Product Information for ZNT8 elisa kit
Description: This Human Zinc transporter 8 (SLC30A8) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC30A8 in samples. An antibody specific for SLC30A8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SLC30A8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC30A8 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC30A8 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: SLC30A8 is expressed at a high level only in the pancreas, particularly in islets of Langerhans. The protein colocalizes with insulin in the secretory pathway granules of the insulin-secreting INS-1 cells. Allelic variants of this gene exist that confer susceptibility to diabetes mellitus, noninsulin-dependent. Several transcript variants encoding different isoforms have been found for this gene.It was also expressed in fetal pancreas, islets of Langerhans, and an insulinoma cell line, but not in HeLa cells. Fluorescence-tagged ZNT8 was expressed in a punctate pattern in the cytoplasm of transfected insulinoma cells and HeLa cells. In insulinoma cells, ZNT8 expression overlapped that of insulin, suggesting that ZNT8 is associated with insulin granule secretion.
Background: SLC30A8 is expressed at a high level only in the pancreas, particularly in islets of Langerhans. The protein colocalizes with insulin in the secretory pathway granules of the insulin-secreting INS-1 cells. Allelic variants of this gene exist that confer susceptibility to diabetes mellitus, noninsulin-dependent. Several transcript variants encoding different isoforms have been found for this gene.It was also expressed in fetal pancreas, islets of Langerhans, and an insulinoma cell line, but not in HeLa cells. Fluorescence-tagged ZNT8 was expressed in a punctate pattern in the cytoplasm of transfected insulinoma cells and HeLa cells. In insulinoma cells, ZNT8 expression overlapped that of insulin, suggesting that ZNT8 is associated with insulin granule secretion.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
zinc transporter 8 isoform a
NCBI Official Synonym Full Names
solute carrier family 30 member 8
NCBI Official Symbol
SLC30A8
NCBI Official Synonym Symbols
ZNT8; ZnT-8
NCBI Protein Information
zinc transporter 8
UniProt Protein Name
Zinc transporter 8
UniProt Gene Name
SLC30A8
UniProt Synonym Gene Names
ZNT8; ZnT-8
UniProt Entry Name
ZNT8_HUMAN
NCBI Description
The protein encoded by this gene is a zinc efflux transporter involved in the accumulation of zinc in intracellular vesicles. This gene is expressed at a high level only in the pancreas, particularly in islets of Langerhans. The encoded protein colocalizes with insulin in the secretory pathway granules of the insulin-secreting INS-1 cells. Allelic variants of this gene exist that confer susceptibility to diabetes mellitus, noninsulin-dependent (NIDDM). Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2010]
Research Articles on ZNT8
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Product Notes
The Human ZNT8 slc30a8 (Catalog #AAA9714270) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714270 ELISA Kit recognizes Human ZNT8. It is sometimes possible for the material contained within the vial of "Zinc Transporter 8 (SLC30A8), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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