Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Zinc transporter 10 (SLC30A10) Standard Curve.)
Mouse Zinc Transporter 10 (SLC30A10) ELISA Kit | ZNT10 elisa kit
Mouse Zinc Transporter 10 (SLC30A10) ELISA Kit
Gene Names
Slc30a10; Gm212; E130106K10Rik
Reactivity
Mouse
Synonyms
Zinc Transporter 10 (SLC30A10); Mouse Zinc Transporter 10 (SLC30A10) ELISA Kit; SLC30A10; DKFZp547M236; ZNT8; ZRC1; ZnT-10; solute carrier family 30 (zinc transporter); member 10; zinc transporter 8; ZNT10 elisa kit
Reactivity
Mouse
Specificity
Mouse Zinc transporter 10 (SLC30A10) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SLC30A10. No significant cross-reactivity or interference between Mouse SLC30A10 and analogues was observed.
Sequence Length
470
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Zinc transporter 10 (SLC30A10) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Zinc transporter 10 (SLC30A10) Standard Curve.)
Related Product Information for ZNT10 elisa kit
Description: This Mouse Zinc transporter 10 (SLC30A10) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC30A10 in samples. An antibody specific for SLC30A10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SLC30A10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC30A10 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC30A10 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Zinc functions as a cofactor for numerous enzymes, nuclear factors, and hormones and as an intra- and intercellular signal ion. Members of the zinc transporter (ZNT)/SLC30 subfamily of the cation diffusion facilitator family, such as SLC30A10, permit cellular efflux of zinc. By searching databases with ZNT sequences, Seve et al. (2004) identified SLC30A10, which they called ZNT10. The deduced protein has a calculated molecular mass of 52.7 kD. It has 6 transmembrane helices, a basic region between helices 4 and 5, and cytoplasmic N and C termini. EST database analysis showed evidence of SLC30A10 expression only in fetal brain and fetal liver.Seve et al. (2004) determined that the SLC30A10 gene contains 4 exons and spans 15 kb.
Background: Zinc functions as a cofactor for numerous enzymes, nuclear factors, and hormones and as an intra- and intercellular signal ion. Members of the zinc transporter (ZNT)/SLC30 subfamily of the cation diffusion facilitator family, such as SLC30A10, permit cellular efflux of zinc. By searching databases with ZNT sequences, Seve et al. (2004) identified SLC30A10, which they called ZNT10. The deduced protein has a calculated molecular mass of 52.7 kD. It has 6 transmembrane helices, a basic region between helices 4 and 5, and cytoplasmic N and C termini. EST database analysis showed evidence of SLC30A10 expression only in fetal brain and fetal liver.Seve et al. (2004) determined that the SLC30A10 gene contains 4 exons and spans 15 kb.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
zinc transporter 10
NCBI Official Synonym Full Names
solute carrier family 30, member 10
NCBI Official Symbol
Slc30a10
NCBI Official Synonym Symbols
Gm212; E130106K10Rik
NCBI Protein Information
zinc transporter 10
UniProt Protein Name
Zinc transporter 10
UniProt Gene Name
Slc30a10
UniProt Synonym Gene Names
Znt10; ZnT-10
Uniprot Description
Plays a pivotal role in manganese transport. Manganese is an essential cation for the function of several enzymes, including some crucially important for the metabolism of neurotransmitters and other neuronal metabolic pathways.
Research Articles on ZNT10
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Product Notes
The Mouse ZNT10 slc30a10 (Catalog #AAA9717036) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717036 ELISA Kit recognizes Mouse ZNT10. It is sometimes possible for the material contained within the vial of "Zinc Transporter 10 (SLC30A10), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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