SDS-PAGE
(Figure annotation denotes ug of protein loaded and % gel used.)
14.3.3 zeta recombinant protein
14.3.3 zeta protein (His tag)
Gene Names
ywhaz-b; ywhaz; ywhazb; 14-3-3z; 14-3-3zeta
Applications
SDS-Page
Purity
> 95% pure
Synonyms
14.3.3 zeta; 14.3.3 zeta protein (His tag); Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein protein; Tyr-3/Trp-5 monooxygenase Activation protein protein; zeta polypeptide protein; Phospholipase A2 protein; Zeta; KCIP-1 protein; 14/3/3 zeta protein; 14-3-3 zeta; Protein kinase C inhibitor protein 1 protein; MGC111427 protein; 14-3-3 zeta protein; 14/3/3 zeta; Tyr3/Trp5 monooxygenase Activation protein protein; zeta polypeptide 14 3 3 protein zeta/delta protein; KCIP1 protein; YWHAZ protein; Zeta protein; 14.3.3 zeta recombinant protein
Host
Human
Purity/Purification
> 95% pure
Form/Format
Supplied as a liquid in PBS, pH 7.4.
Concentration
1 mg/ml (varies by lot)
Sequence
1-245 amino acids: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMDK NELVQKAKLA EQAERYDDMA ACMKSVTEQG AELSNEERNL LSVAYKNVVG ARRSSWRVVS SIEQKTEGAE KKQQMAREYR EKIETELRDI CNDVLSLLEK FLIPNASQAE SKVFYLKMKG DYYRYLAEVA AGDDKKGIVD QSQQAYQEAF EISKKEMQPT HPIRLGLALN FSVFYYEILN SPEKACSLAK TAFDEAIAEL DTLSEESYKD STLIMQLLRD NLTLWTSDTQ GDEAEAGEGG EN
Sequence Length
245
Applicable Applications for 14.3.3 zeta recombinant protein
SDS-PAGE
Protein Type
Recombinant
Biological Significance
The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon,sigma, zeta, tau and eta that have been identified in mammals. 14-3-3 zeta interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Recombinant human YWHAZ, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Expression System
E Coli
Tag/Conjugate
His tag
Preparation and Storage
Store at 4 degree C for short term storage (1/2 weeks). Aliquot and store at -20 degree C or - 70 degree C for long term storage. Avoid repeated freeze/thaw cycles.
SDS-PAGE
(Figure annotation denotes ug of protein loaded and % gel used.)
SDS-PAGE
(Figure annotation denotes ug of protein loaded and % gel used.)
Related Product Information for 14.3.3 zeta recombinant protein
Purified recombinant Human 14.3.3 zeta protein
Product Categories/Family for 14.3.3 zeta recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
32 kDa (245 aa), confirmed by MALDI-TOF.
NCBI Official Full Name
14-3-3 protein zeta
NCBI Official Synonym Full Names
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta<
NCBI Official Symbol
ywhaz-b
NCBI Official Synonym Symbols
ywhaz; ywhazb; 14-3-3z; 14-3-3zeta
NCBI Protein Information
14-3-3 protein zeta
UniProt Protein Name
14-3-3 protein zeta
UniProt Gene Name
ywhaz
UniProt Entry Name
1433Z_XENLA
Uniprot Description
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
Research Articles on 14.3.3 zeta
Similar Products
Product Notes
The 14.3.3 zeta ywhaz (Catalog #AAA836040) is a Recombinant Protein produced from Human and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's 14.3.3 zeta can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the 14.3.3 zeta ywhaz for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: 1-245 amino acids: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMDK NELVQKAKLA EQAERYDDMA ACMKSVTEQG AELSNEERNL LSVAYKNVVG ARRSSWRVVS SIEQKTEGAE KKQQMAREYR EKIETELRDI CNDVLSLLEK FLIPNASQAE SKVFYLKMKG DYYRYLAEVA AGDDKKGIVD QSQQAYQEAF EISKKEMQPT HPIRLGLALN FSVFYYEILN SPEKACSLAK TAFDEAIAEL DTLSEESYKD STLIMQLLRD NLTLWTSDTQ GDEAEAGEGG EN. It is sometimes possible for the material contained within the vial of "14.3.3 zeta, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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