Rabbit anti-Human OCT4 Polyclonal Antibody | anti-OCT4 antibody

OCT4 (E125) (POU5F1, OCT3, OCT4, OTF3, POU domain, class 5, transcription factor 1, Octamer-binding protein 3, Octamer-binding protein 4, Octamer-binding transcription factor 3) (Biotin)

Cat. Num. AAA6327570

• Gene Names: POU5F1; OCT3; OCT4; OTF3; OTF4; OTF-3; Oct-3; Oct-4
• Reactivity: Human
• Applications: Immunofluorescence, ELISA, Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: OCT4; Polyclonal Antibody; OCT4 (E125) (POU5F1; OCT3; OTF3; POU domain; class 5; transcription factor 1; Octamer-binding protein 3; Octamer-binding protein 4; Octamer-binding transcription factor 3) (Biotin); POU5F1; Octamer-binding transcription factor 3; anti-OCT4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-OCT4 antibody: Immunofluorescence (IF), ELISA (EIA), Western Blot (WB)
• Application Notes: IF: 1:10-50; ELISA: 1:1,000; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: OCT4 (OCT3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 118~148 amino acids surrounding E125 of human OCT3/4.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of OCT3/4 Antibody (E125) in HL60 cell line lysates (35ug/lane). OCT3/4 (arrow) was detected using the purified Pab.)

Immunofluorescence (IF)

(Fluorescent confocal image of SY5Y cells stained with AP2046c OCT4 (E125) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with AP2046c OCT4 (E125) primary antibody (1:500, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 uM, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 3 min). OCT4 immunoreactivity is localized mainly to the nuclei and also to the cytoplasm.)

Related Product Information for anti-OCT4 antibody

Transcription factors containing the POU homeodomain have been shown to be important regulators of tissue-specific gene expression in lymphoid and pituitary differentiation and in early mammalian development. Two forms of OCT3 mRNA are expressed in adult tissues as a result of alternative splicing--OCT3A and OCT3B. Reverse transcriptase PCR showed low level of expression in both OCT3A and OCT3B mRNA in all adult human tissues examined. Oct3 is present in mouse oocytes before and after fertilization. When fertilized oocytes were injected with antisense Oct3 oligonucleotides or double-stranded DNA containing the octamer motif, embryonic DNA synthesis was inhibited and the embryos were arrested at the one-cell stage.

Product Categories/Family for anti-OCT4 antibody

Antibodies; Transcription Factors

References

Looijenga, L.H., et al., Cancer Res. 63(9):2244-2250 (2003). Remenyi, A., et al., Genes Dev. 17(16):2048-2059 (2003). Nichols, J., et al., Cell 95(3):379-391 (1998). Crouau-Roy, B., et al., Genomics 21(1):241-243 (1994). Takeda, J., et al., Nucleic Acids Res. 20(17):4613-4620 (1992).

NCBI and Uniprot Product Information

• NCBI GI #: 42560248
• NCBI GeneID: 5460
• NCBI Accession #: NP_002692.2
• NCBI GenBank Nucleotide #: NM_002701.4
• UniProt Accession #: Q01860; P31359; Q15167; Q15168; Q16422; Q5STF3; Q5STF4; A6NCS1; A6NLL8; D2IYK4
• Molecular Weight: 38,571 Da
• NCBI Official Full Name: POU domain, class 5, transcription factor 1 isoform 1
• NCBI Official Synonym Full Names: POU class 5 homeobox 1
• NCBI Official Symbol: POU5F1
• NCBI Official Synonym Symbols: OCT3; OCT4; OTF3; OTF4; OTF-3; Oct-3; Oct-4
• NCBI Protein Information: POU domain, class 5, transcription factor 1; octamer-binding protein 3; octamer-binding protein 4; POU domain transcription factor OCT4; octamer-binding transcription factor 3; octamer-binding transcription factor-3; POU-type homeodomain-containing DNA-bi
• UniProt Protein Name: POU domain, class 5, transcription factor 1
• UniProt Gene Name: POU5F1
• UniProt Synonym Gene Names: OCT3; OCT4; OTF3; Oct-3; Oct-4; OTF-3
• UniProt Entry Name: PO5F1_HUMAN

NCBI Description

This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq, Mar 2010]

Uniprot Description

Oct4: Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. Interacts with UBE2I and ZSCAN10. Interacts with PKM2. Interacts with WWP2. Transcriptional activity is positively regulated by PKM2. Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues. Belongs to the POU transcription factor family. Class- 5 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 6p21.31

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; miRNA binding; DNA binding; sequence-specific DNA binding; ubiquitin protein ligase binding; transcription factor binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; blastocyst development; anatomical structure morphogenesis; regulation of asymmetric cell division; regulation of transcription, DNA-dependent; regulation of gene expression; somatic stem cell maintenance; endodermal cell fate specification; response to wounding; positive regulation of transcription from RNA polymerase II promoter; mRNA transcription from RNA polymerase II promoter