Rat Xanthine dehydrogenase/oxidase ELISA Kit | Xdh elisa kit

Rat Xanthine dehydrogenase/oxidase ELISA Kit

Cat. Num. AAA761951

• Gene Names: XDH; XO; XOR
• Reactivity: Rat
• Synonyms: Xanthine dehydrogenase/oxidase; Rat Xanthine dehydrogenase/oxidase ELISA Kit; Xdh; Xdh elisa kit

• Reactivity: Rat
• Sequence Length: 1333

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Xdh elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat Xdh antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat Xdh antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat Xdh amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofRat Xdh can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 91823271
• NCBI GeneID: 7498
• NCBI Accession #: NP_000370.2
• NCBI GenBank Nucleotide #: NM_000379.3
• Molecular Weight: 146,424 Da
• NCBI Official Full Name: xanthine dehydrogenase/oxidase
• NCBI Official Synonym Full Names: xanthine dehydrogenase
• NCBI Official Symbol: XDH
• NCBI Official Synonym Symbols: XO; XOR
• NCBI Protein Information: xanthine dehydrogenase/oxidase
• UniProt Protein Name: Xanthine dehydrogenase/oxidase
• Protein Family: Xanthine dehydrogenase
• UniProt Gene Name: XDH
• UniProt Synonym Gene Names: XDHA; XD; XO; XOR
• UniProt Entry Name: XDH_HUMAN

NCBI Description

Xanthine dehydrogenase belongs to the group of molybdenum-containing hydroxylases involved in the oxidative metabolism of purines. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Xanthine dehydrogenase can be converted to xanthine oxidase by reversible sulfhydryl oxidation or by irreversible proteolytic modification. Defects in xanthine dehydrogenase cause xanthinuria, may contribute to adult respiratory stress syndrome, and may potentiate influenza infection through an oxygen metabolite-dependent mechanism. [provided by RefSeq, Jan 2014]

Uniprot Description

XDH: Key enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid. Contributes to the generation of reactive oxygen species. Has also low oxidase activity towards aldehydes (in vitro). Defects in XDH are the cause of xanthinuria type 1 (XU1). Xanthinuria is characterized by excretion of very large amounts of xanthine in the urine and a tendency to form xanthine stones. Uric acid is strikingly diminished in serum and urine. XU1 is due to isolated xanthine dehydrogenase. XU1 patients can metabolize allopurinol. Belongs to the xanthine dehydrogenase family.

Protein type: Secondary Metabolites Metabolism - caffeine; EC 1.17.3.2; Xenobiotic Metabolism - drug metabolism - other enzymes; EC 1.17.1.4; Nucleotide Metabolism - purine; Oxidoreductase

Chromosomal Location of Human Ortholog: 2p23.1

Cellular Component: cytosol; extracellular space; peroxisome; sarcoplasmic reticulum

Molecular Function: 2 iron, 2 sulfur cluster binding; electron carrier activity; FAD binding; iron ion binding; molybdopterin cofactor binding; oxidoreductase activity, acting on CH-OH group of donors; oxidoreductase activity, acting on the aldehyde or oxo group of donors; protein homodimerization activity; xanthine dehydrogenase activity; xanthine oxidase activity

Biological Process: caspase activation; lactation; negative regulation of endothelial cell differentiation; negative regulation of endothelial cell proliferation; negative regulation of protein amino acid phosphorylation; negative regulation of protein kinase B signaling cascade; nucleobase, nucleoside and nucleotide metabolic process; purine base metabolic process; purine nucleotide catabolic process; xanthine catabolic process

Disease: Xanthinuria, Type I