Typical Testing Data/Standard Curve (for reference only)
Human Xanthine dehydrogenase/oxidase ELISA Kit | XDH elisa kit
Human Xanthine dehydrogenase/oxidase ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Xanthine dehydrogenase/oxidase. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Xanthine dehydrogenase/oxidase and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Xanthine dehydrogenase/oxidase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Xanthine dehydrogenase/oxidase in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
Xanthine dehydrogenase belongs to the group of molybdenum-containing hydroxylases involved in the oxidative metabolism of purines. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Xanthine dehydrogenase can be converted to xanthine oxidase by reversible sulfhydryl oxidation or by irreversible proteolytic modification. Defects in xanthine dehydrogenase cause xanthinuria, may contribute to adult respiratory stress syndrome, and may potentiate influenza infection through an oxygen metabolite-dependent mechanism. [provided by RefSeq, Jan 2014]
Uniprot Description
XDH: Key enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid. Contributes to the generation of reactive oxygen species. Has also low oxidase activity towards aldehydes (in vitro). Defects in XDH are the cause of xanthinuria type 1 (XU1). Xanthinuria is characterized by excretion of very large amounts of xanthine in the urine and a tendency to form xanthine stones. Uric acid is strikingly diminished in serum and urine. XU1 is due to isolated xanthine dehydrogenase. XU1 patients can metabolize allopurinol. Belongs to the xanthine dehydrogenase family.
Protein type: Nucleotide Metabolism - purine; Xenobiotic Metabolism - drug metabolism - other enzymes; Secondary Metabolites Metabolism - caffeine; EC 1.17.1.4; Oxidoreductase; EC 1.17.3.2
Chromosomal Location of Human Ortholog: 2p23.1
Cellular Component: cytosol; extracellular space; peroxisome; sarcoplasmic reticulum
Molecular Function: 2 iron, 2 sulfur cluster binding; electron carrier activity; FAD binding; iron ion binding; molybdopterin cofactor binding; oxidoreductase activity, acting on CH-OH group of donors; oxidoreductase activity, acting on the aldehyde or oxo group of donors; protein homodimerization activity; xanthine dehydrogenase activity; xanthine oxidase activity
Biological Process: caspase activation; lactation; negative regulation of endothelial cell differentiation; negative regulation of endothelial cell proliferation; negative regulation of protein amino acid phosphorylation; negative regulation of protein kinase B signaling cascade; nucleobase, nucleoside and nucleotide metabolic process; purine base metabolic process; purine nucleotide catabolic process; xanthine catabolic process
Disease: Xanthinuria, Type I
Research Articles on XDH
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Product Notes
The Human XDH xdh (Catalog #AAA2885435) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2885435 ELISA Kit recognizes Human XDH. It is sometimes possible for the material contained within the vial of "Xanthine dehydrogenase/oxidase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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