Porcine Xanthine dehydrogenase/oxidase [Includes: Xanthine dehydrogenase (XDH) ELISA Kit | XDH elisa kit

Porcine Xanthine dehydrogenase/oxidase [Includes: Xanthine dehydrogenase (XDH) ELISA Kit

Cat. Num. AAA7213298

• Gene Names: XDH; XO; XOR
• Reactivity: Porcine
• Synonyms: Xanthine dehydrogenase/oxidase [Includes: Xanthine dehydrogenase (XDH); Porcine Xanthine dehydrogenase/oxidase [Includes: Xanthine dehydrogenase (XDH) ELISA Kit; XDH elisa kit

• Reactivity: Porcine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This XDH ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine XDH. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for XDH elisa kit

Principle of the assay: XDH ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-XDH antibody and an XDH-HRP conjugate. The assay sample and buffer are incubated together with XDH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the XDH concentration since XDH from samples and XDH-HRP conjugate compete for the anti-XDH antibody binding site. Since the number of sites is limited, as more sites are occupied by XDH from the sample, fewer sites are left to bind XDH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The XDH concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 91823271
• NCBI GeneID: 7498
• NCBI Accession #: NP_000370.2
• NCBI GenBank Nucleotide #: NM_000379.3
• Molecular Weight: 146,424 Da
• NCBI Official Full Name: xanthine dehydrogenase/oxidase
• NCBI Official Synonym Full Names: xanthine dehydrogenase
• NCBI Official Symbol: XDH
• NCBI Official Synonym Symbols: XO; XOR
• NCBI Protein Information: xanthine dehydrogenase/oxidase; xanthine oxidoreductase
• UniProt Protein Name: Xanthine dehydrogenase/oxidase
• Protein Family: Xanthine dehydrogenase
• UniProt Gene Name: XDH
• UniProt Synonym Gene Names: XDHA; XD; XO; XOR
• UniProt Entry Name: XDH_HUMAN

NCBI Description

Xanthine dehydrogenase belongs to the group of molybdenum-containing hydroxylases involved in the oxidative metabolism of purines. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Xanthine dehydrogenase can be converted to xanthine oxidase by reversible sulfhydryl oxidation or by irreversible proteolytic modification. Defects in xanthine dehydrogenase cause xanthinuria, may contribute to adult respiratory stress syndrome, and may potentiate influenza infection through an oxygen metabolite-dependent mechanism. [provided by RefSeq, Jan 2014]

Uniprot Description

XDH: Key enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid. Contributes to the generation of reactive oxygen species. Has also low oxidase activity towards aldehydes (in vitro). Defects in XDH are the cause of xanthinuria type 1 (XU1). Xanthinuria is characterized by excretion of very large amounts of xanthine in the urine and a tendency to form xanthine stones. Uric acid is strikingly diminished in serum and urine. XU1 is due to isolated xanthine dehydrogenase. XU1 patients can metabolize allopurinol. Belongs to the xanthine dehydrogenase family.

Protein type: Oxidoreductase; EC 1.17.1.4; Xenobiotic Metabolism - drug metabolism - other enzymes; EC 1.17.3.2; Secondary Metabolites Metabolism - caffeine; Nucleotide Metabolism - purine

Chromosomal Location of Human Ortholog: 2p23.1

Cellular Component: extracellular space; peroxisome; cytosol

Molecular Function: 2 iron, 2 sulfur cluster binding; molybdopterin cofactor binding; UDP-N-acetylmuramate dehydrogenase activity; protein homodimerization activity; oxidoreductase activity, acting on the aldehyde or oxo group of donors; electron carrier activity; FAD binding; iron ion binding; xanthine dehydrogenase activity; xanthine oxidase activity

Biological Process: caspase activation; lactation; xanthine catabolic process; negative regulation of protein kinase B signaling cascade; negative regulation of endothelial cell differentiation; negative regulation of protein amino acid phosphorylation; nucleobase, nucleoside and nucleotide metabolic process; negative regulation of endothelial cell proliferation; purine nucleotide catabolic process; purine base metabolic process

Disease: Xanthinuria, Type I