Human xanthine dehydrogenase ELISA Kit | XDH elisa kit

Human Xanthine Oxidase, XOD ELISA Kit

Cat. Num. AAA701269

• Gene Names: XDH; XO; XOR
• Reactivity: Human
• Synonyms: xanthine dehydrogenase; Human Xanthine Oxidase; XOD ELISA Kit; Human Xanthine Oxidase (XOD) ELISA Kit; XO; XOR; xanthene dehydrogenase; xanthine oxidase; xanthine oxidoreductase; XDH elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human XDH. No significant cross-reactivity or interference between Human XDH and analogues was observed.
• Sequence Length: 1333

• Samples: Serum, plasma, urine, tissue homogenates
• Detection Range: 0.156 ng/ml-10 ng/ml
• Sensitivity: 0.039 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for XDH elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to XOD. Standards or samples are then added to the appropriate microtiter plate wells with a biotin conjugated antibody preparation specific for XOD and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain XOD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of XOD in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 91823271
• NCBI GeneID: 7498
• NCBI Accession #: NP_000370.2
• NCBI GenBank Nucleotide #: NM_000379.3
• UniProt Accession #: P47989; Q16681; Q16712; Q4PJ16
• Molecular Weight: 146,424 Da
• NCBI Official Full Name: xanthine dehydrogenase/oxidase
• NCBI Official Synonym Full Names: xanthine dehydrogenase
• NCBI Official Symbol: XDH
• NCBI Official Synonym Symbols: XO; XOR
• NCBI Protein Information: xanthine dehydrogenase/oxidase; xanthine oxidoreductase
• UniProt Protein Name: Xanthine dehydrogenase/oxidase
• Protein Family: Xanthine dehydrogenase
• UniProt Gene Name: XDH
• UniProt Synonym Gene Names: XDHA; XD; XO; XOR
• UniProt Entry Name: XDH_HUMAN

NCBI Description

Xanthine dehydrogenase belongs to the group of molybdenum-containing hydroxylases involved in the oxidative metabolism of purines. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Xanthine dehydrogenase can be converted to xanthine oxidase by reversible sulfhydryl oxidation or by irreversible proteolytic modification. Defects in xanthine dehydrogenase cause xanthinuria, may contribute to adult respiratory stress syndrome, and may potentiate influenza infection through an oxygen metabolite-dependent mechanism. [provided by RefSeq, Jan 2014]

Uniprot Description

Function: Key enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid. Contributes to the generation of reactive oxygen species. Has also low oxidase activity towards aldehydes (in vitro).1 PublicationManual assertion based on experiment in:Ref.9

Catalytic activity: Hypoxanthine + NAD+ + H2O = xanthine + NADH.2 PublicationsManual assertion based on experiment in:Ref.4

Cofactor: Binds 2 2Fe-2S clusters.1 PublicationManual assertion based on experiment in:Ref.9

Enzyme regulation: Can be converted from the dehydrogenase form (D) to the oxidase form (O) irreversibly by proteolysis or reversibly through the oxidation of sulfhydryl groups 1 PublicationManual assertion based on experiment in:Ref.10