Human Protein Wnt-7a ELISA Kit | WNT7A elisa kit

Human Protein Wnt-7a ELISA Kit

Cat. Num. AAA764320

• Reactivity: Human
• Synonyms: Protein Wnt-7a; Human Protein Wnt-7a ELISA Kit; WNT7A; WNT7A elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of WNT7A. No significant cross-reactivity or interference between WNT7A and analogues was observed.
• Sequence Length: 349

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 31.25-2000pg/ml
• Sensitivity: 18.75pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for WNT7A elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 17505191
• NCBI GeneID: 7476
• NCBI Accession #: NP_004616.2
• NCBI GenBank Nucleotide #: NM_004625.3
• Molecular Weight: 39,005 Da
• NCBI Official Full Name: protein Wnt-7a
• NCBI Official Synonym Full Names: wingless-type MMTV integration site family member 7A
• NCBI Official Symbol: WNT7A
• NCBI Protein Information: protein Wnt-7a
• UniProt Protein Name: Protein Wnt-7a
• Protein Family: Protein
• UniProt Gene Name: WNT7A
• UniProt Entry Name: WNT7A_HUMAN

NCBI Description

This gene is a member of the WNT gene family, which consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is involved in the development of the anterior-posterior axis in the female reproductive tract, and also plays a critical role in uterine smooth muscle pattering and maintenance of adult uterine function. Mutations in this gene are associated with Fuhrmann and Al-Awadi/Raas-Rothschild/Schinzel phocomelia syndromes. [provided by RefSeq, Jul 2008]

Uniprot Description

WNT7A: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. Signaling by Wnt-7a allows sexually dimorphic development of the mullerian ducts. Defects in WNT7A are the cause of limb pelvis hypoplasia aplasia syndrome (LPHAS). A syndrome of severe deficiency of the extremities due to hypo- or aplasia of one or more long bones of one or more limbs. Pelvic manifestations include hip dislocation, hypoplastic iliac bone and aplastic pubic bones. Thoracic deformity, unusual facies and genitourinary anomalies can be present. Defects in WNT7A are a cause of Fuhrmann syndrome (FUHRS); also known as fibular aplasia or hypoplasia femoral bowing and poly- syn- and oligodactyly. Fuhrmann syndrome is a distinct limb-malformation disorder characterized also by various degrees of limb aplasia/hypoplasia and joint dysplasia. Belongs to the Wnt family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3p25

Cellular Component: cell surface; endoplasmic reticulum lumen; extracellular region; extracellular space; Golgi lumen; plasma membrane; proteinaceous extracellular matrix

Molecular Function: cytokine activity; frizzled binding; protein binding; receptor agonist activity; receptor binding

Biological Process: angiogenesis; asymmetric protein localization; axonogenesis; cartilage condensation; cell fate commitment; cell proliferation in forebrain; central nervous system vasculogenesis; cerebellar granule cell differentiation; chondrocyte differentiation; dorsal/ventral pattern formation; embryonic axis specification; embryonic digit morphogenesis; embryonic forelimb morphogenesis; embryonic hindlimb morphogenesis; establishment of cell polarity; fallopian tube development; negative regulation of apoptosis; negative regulation of neurogenesis; neuron differentiation; neurotransmitter secretion; palate development; positive regulation of epithelial cell proliferation involved in wound healing; positive regulation of JNK cascade; positive regulation of synaptogenesis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of axon diameter; response to estradiol stimulus; satellite cell activation; satellite cell compartment self-renewal involved in skeletal muscle regeneration; sex differentiation; somatic stem cell division; somatic stem cell maintenance; stem cell development; synaptogenesis; Wnt receptor signaling pathway through beta-catenin

Disease: Fibular Aplasia Or Hypoplasia, Femoral Bowing And Poly-, Syn-, And Oligodactyly; Ulna And Fibula, Absence Of, With Severe Limb Deficiency