Mouse Protein Wnt-7a (WNT7A) ELISA Kit | WNT7A elisa kit

Mouse Protein Wnt-7a (WNT7A) ELISA Kit

Cat. Num. AAA1601337

• Reactivity: Mouse
• Synonyms: Protein Wnt-7a (WNT7A); Mouse Protein Wnt-7a (WNT7A) ELISA Kit; WNT7A elisa kit

• Reactivity: Mouse
• Sequence Length: 349

• Samples: Serum, plasma, cell culture supernates, ascites, tissue homogenates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.03-9ng/ml
• Sensitivity: 0.012ng/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for WNT7A elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of Mouse Protein Wnt-7a (also known as WNT7A) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.

Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse WNT7A antibody. WNT7A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse WNT7A Antibody is added and binds to WNT7A in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated WNT7A antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse WNT7A. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 17505191
• NCBI GeneID: 7476
• NCBI Accession #: NP_004616.2
• NCBI GenBank Nucleotide #: NM_004625.3
• UniProt Accession #: O00755; Q96H90; Q9Y560
• Molecular Weight: 39,005 Da
• NCBI Official Full Name: protein Wnt-7a
• NCBI Official Synonym Full Names: Wnt family member 7A
• NCBI Official Symbol: WNT7A
• NCBI Protein Information: protein Wnt-7a
• UniProt Protein Name: Protein Wnt-7a
• UniProt Gene Name: WNT7A

NCBI Description

This gene is a member of the WNT gene family, which consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is involved in the development of the anterior-posterior axis in the female reproductive tract, and also plays a critical role in uterine smooth muscle pattering and maintenance of adult uterine function. Mutations in this gene are associated with Fuhrmann and Al-Awadi/Raas-Rothschild/Schinzel phocomelia syndromes. [provided by RefSeq, Jul 2008]

Uniprot Description

Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt/beta-catenin signaling pathway (). Plays an important role in embryonic development, including dorsal versus ventral patterning during limb development, skeleton development and urogenital tract development (PubMed:16826533). Required for normal, sexually dimorphic development of the Mullerian ducts, and for normal fertility in both sexes. Required for normal neural stem cell proliferation in the hippocampus dentate gyrus. Required for normal progress through the cell cycle in neural progenitor cells, for self-renewal of neural stem cells, and for normal neuronal differentiation and maturation. Promotes formation of synapses via its interaction with FZD5 ().